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Literature summary for 3.4.24.70 extracted from

  • Anu, P.; Madanan, M.; Nair, A.; Nair, G.; Nair, G.; Sudhakaran, P.; Satheeshkumar, P.
    Heterologous expression, purification and characterization of an oligopeptidase A from the pathogen Leptospira interrogans (2018), Mol. Biotechnol., 60, 302-309 .
    View publication on PubMed
Search for more literature for 3.4.24.70

Cloned(Commentary)

Cloned (Comment) Organism
gene prlC, recombinant expression of N-terminally His-tagged enzyme in Escherichia ocli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha Leptospira interrogans serovar Copenhageni

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ about 75% inhibition at 1 mM Leptospira interrogans serovar Copenhageni
Fe3+ about 90% inhibition at 1 mM Leptospira interrogans serovar Copenhageni
Mg2+ about 75% inhibition at 1 mM Leptospira interrogans serovar Copenhageni
Na+ about 90% inhibition at 1 mM Leptospira interrogans serovar Copenhageni
Na-EDTA 50% inhibition at 10 mM Leptospira interrogans serovar Copenhageni
phenylmethylsulfonyl fluoride PMSF, 80% inhibition at 1 mM Leptospira interrogans serovar Copenhageni
trans-epoxysuccinyl L-leucylamido-(4-guanidino)butane E-64, 25% inhibition at 0.01 mM Leptospira interrogans serovar Copenhageni

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ can substitute for Zn2+ Leptospira interrogans serovar Copenhageni
additional information OpdA is a metalloproteinase Leptospira interrogans serovar Copenhageni
Zn2+ required, activates Leptospira interrogans serovar Copenhageni

Organism

Organism UniProt Comment Textmining
Leptospira interrogans serovar Copenhageni Q72SL8
-
-
Leptospira interrogans serovar Copenhageni Fiocruz L1-130 Q72SL8
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia ocli strain BL21(DE3) by nickel affinity chromatography Leptospira interrogans serovar Copenhageni

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
azocasein + H2O
-
 Leptospira interrogans serovar Copenhageni ?
-
 ?
azocasein + H2O
-
 Leptospira interrogans serovar Copenhageni Fiocruz L1-130 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 71000, recombinant N-terminally His-tagged enzyme, SDS-PAGE Leptospira interrogans serovar Copenhageni

Synonyms

Synonyms Comment Organism
OpdA
-
 Leptospira interrogans serovar Copenhageni
prlC
-
 Leptospira interrogans serovar Copenhageni

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 recombinant enzyme Leptospira interrogans serovar Copenhageni

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 recombinant enzyme Leptospira interrogans serovar Copenhageni