Literature summary for 3.4.24.70 extracted from

Anu, P.; Madanan, M.; Nair, A.; Nair, G.; Nair, G.; Sudhakaran, P.; Satheeshkumar, P.
Heterologous expression, purification and characterization of an oligopeptidase A from the pathogen Leptospira interrogans (2018), Mol. Biotechnol., 60, 302-309 .

Search for more literature for 3.4.24.70

Cloned(Commentary)

Cloned (Comment)	Organism
gene prlC, recombinant expression of N-terminally His-tagged enzyme in Escherichia ocli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha	Leptospira interrogans serovar Copenhageni

Inhibitors

Inhibitors	Comment	Organism	Structure
Ca2+	about 75% inhibition at 1 mM	Leptospira interrogans serovar Copenhageni
Fe3+	about 90% inhibition at 1 mM	Leptospira interrogans serovar Copenhageni
Mg2+	about 75% inhibition at 1 mM	Leptospira interrogans serovar Copenhageni
Na+	about 90% inhibition at 1 mM	Leptospira interrogans serovar Copenhageni
Na-EDTA	50% inhibition at 10 mM	Leptospira interrogans serovar Copenhageni
phenylmethylsulfonyl fluoride	PMSF, 80% inhibition at 1 mM	Leptospira interrogans serovar Copenhageni
trans-epoxysuccinyl L-leucylamido-(4-guanidino)butane	E-64, 25% inhibition at 0.01 mM	Leptospira interrogans serovar Copenhageni

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Cu2+	can substitute for Zn2+	Leptospira interrogans serovar Copenhageni
additional information	OpdA is a metalloproteinase	Leptospira interrogans serovar Copenhageni
Zn2+	required, activates	Leptospira interrogans serovar Copenhageni

Organism

Organism	UniProt	Comment	Textmining
Leptospira interrogans serovar Copenhageni	Q72SL8	-	-
Leptospira interrogans serovar Copenhageni Fiocruz L1-130	Q72SL8	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged enzyme from Escherichia ocli strain BL21(DE3) by nickel affinity chromatography	Leptospira interrogans serovar Copenhageni

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
azocasein + H2O	-	Leptospira interrogans serovar Copenhageni	?	-	?
azocasein + H2O	-	Leptospira interrogans serovar Copenhageni Fiocruz L1-130	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 71000, recombinant N-terminally His-tagged enzyme, SDS-PAGE	Leptospira interrogans serovar Copenhageni

Synonyms

Synonyms	Comment	Organism
OpdA	-	Leptospira interrogans serovar Copenhageni
prlC	-	Leptospira interrogans serovar Copenhageni

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	recombinant enzyme	Leptospira interrogans serovar Copenhageni

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	recombinant enzyme	Leptospira interrogans serovar Copenhageni

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Release 2023.1 (January 2023)