Application | Comment | Organism |
---|---|---|
drug development | the botulinum toxin is a target for development of specific drugs to treat botulism | Clostridium botulinum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | construction of type A-specific monoclonal antibodies using antigene heavy chain antibody fragment VH/VHH from a nonimmune camel, primers specific to human VH gene segments, and recombinant expression in phagemid-transformed Escherichia coli. The selected antibodies inhibiting the endopeptidase activity of BoTxA light chain and of botulism in hosts in vivo. Molecular docking and interface binding of BoTxA/LC and antibody VHH17 | Clostridium botulinum | |
PTEN | a zinc-chelating agent | Clostridium botulinum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | zinc-dependent metalloprotease | Clostridium botulinum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
x * 50000, type A botulinum neurotoxin light chain, SDS-PAGE | Clostridium botulinum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
SNAP-25 + H2O | Clostridium botulinum | i.e. 25-kDa synaptosome-associated protein | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium botulinum | P10845 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
SNAP-25 + H2O | i.e. 25-kDa synaptosome-associated protein | Clostridium botulinum | ? | - |
? | |
SNRTRIDEANK(Dnp)RA(S-(N-[4-methyl-7-dimethylamino-coumarin-3-yl]-carboxamidomethyl)-L-cysteine)RML + H2O | a synthetic fluorogenic peptide substrate of BoTxA/LC, representing amino acid residues 187-203 of SNAP25, a cleavage site of the enzyme | Clostridium botulinum | SNRTRIDEANK(Dnp) + RA(S-(N-[4-methyl-7-dimethylamino-coumarin-3-yl]-carboxamidomethyl)-L-cysteine)RML | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 50000, type A botulinum neurotoxin light chain, SDS-PAGE | Clostridium botulinum |
Synonyms | Comment | Organism |
---|---|---|
botulinum neurotoxin type A | - |
Clostridium botulinum |
BoTxA | - |
Clostridium botulinum |
type A botulinum neurotoxin | - |
Clostridium botulinum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Clostridium botulinum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Clostridium botulinum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | binding and inhibition kinetics of antigene heavy chain antibody fragment VH/VHH-specific antibodies, overview | Clostridium botulinum |