Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Saccharomyces cerevisiae | 5739 | - |
additional information | Nfs1 needs to be processed by MPP to be functional in the mitochondrion and the nucleus, the latter case requires a mechanism that involves reverse translocation of processed Nfs1, i.e. the retrograde movement of Nfs1 | Saccharomyces cerevisiae | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Nfs1 + H2O | Saccharomyces cerevisiae | MPP cleaves the precursor between Phe33 and Tyr34, Nfs1 processing, overview | processed Nfs1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
several strains, overview | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | construction of a mutant MPP recognition sequence in substrate Nfs1 by replacing the two arginine codons at positions -2 and -3 prevents cleavage by MPP | Saccharomyces cerevisiae | ? | - |
? | |
Nfs1 + H2O | MPP cleaves the precursor between Phe33 and Tyr34, Nfs1 processing, overview | Saccharomyces cerevisiae | processed Nfs1 | - |
? | |
Nfs1 + H2O | recombinant His-tagged substrate. Nfs1 is a highly conserved mitochondrial cysteine desulfurase with dual localization in mitochondria and nuclei, mechanism of Nfs1 distribution, overview | Saccharomyces cerevisiae | processed Nfs1 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MPP | - |
Saccharomyces cerevisiae |
General Information | Comment | Organism |
---|---|---|
metabolism | yeast Nfs1 undergoes two steps of proteolytic processing: first it is cleaved by the mitochondrial processing peptidase, MPP, which removes its mitochondrial targeting sequence, and then it is cleaved by a peptidase, designated Icp55, which removes three amino acids from its N-terminus | Saccharomyces cerevisiae |