Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Rattus norvegicus |
rat, alpha- and beta-subunit, expressed in Escherichia coli as fusion proteins with maltose-binding protein | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Rattus norvegicus | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
1 * 50000 (beta-MPP) + 1 * 53000 (alpha-MPP), recombinant enzyme, SDS-PAGE | Rattus norvegicus |
53000 | - |
1 * 50000 (beta-MPP) + 1 * 53000 (alpha-MPP), recombinant enzyme, SDS-PAGE | Rattus norvegicus |
108000 | - |
gel filtration | Rattus norvegicus |
108000 | - |
rat | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
alpha- and beta-subunit, isolated as recombinant fusion proteins with maltose-binding protein from Escherichia coli, treated with factor Xa, then separatly purified to homogeneity, active MPP recovered from mixed subunits after denaturation/renaturation procedure | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
mitochondrial malate dehydrogenase precursor + H2O | from rat | Rattus norvegicus | mitochondrial malate dehydrogenase + mitochondrial malate dehydrogenase transit peptide | - |
? | |
Ornithine carbamoyl transferase precursor + H2O | EC 2.1.3.3, from rat | Rattus norvegicus | Ornithine carbamoyl transferase intermediate form | - |
? | |
Rieske FES protein precursor + H2O | from Neurospora | Rattus norvegicus | Rieske FES protein intermediate form | from Neurospora | ? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 50000 (beta-MPP) + 1 * 53000 (alpha-MPP), recombinant enzyme, SDS-PAGE | Rattus norvegicus |