Cloned (Comment) | Organism |
---|---|
- |
Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
A140D | specific activity with the substrates (7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH and (7-methoxycoumarin-4-yl)acetyl-QKLVFFAEDVK(2,4-dinitrophenyl)-OH is below 1 nmol/min*mg | Homo sapiens |
A140F | specific activity with the substrates (7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH and (7-methoxycoumarin-4-yl)acetyl-QKLVFFAEDVK(2,4-dinitrophenyl)-OH is below 1 nmol/min*mg | Homo sapiens |
A140K | specific activity with the substrates (7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH and (7-methoxycoumarin-4-yl)acetyl-QKLVFFAEDVK(2,4-dinitrophenyl)-OH is below 1 nmol/min*mg | Homo sapiens |
A140N | specific activity with the substrates (7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH and (7-methoxycoumarin-4-yl)acetyl-QKLVFFAEDVK(2,4-dinitrophenyl)-OH is below 1 nmol/min*mg | Homo sapiens |
A140W | specific activity with the substrates (7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH and (7-methoxycoumarin-4-yl)acetyl-QKLVFFAEDVK(2,4-dinitrophenyl)-OH is below 1 nmol/min*mg | Homo sapiens |
A140Y | specific activity with the substrates (7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH and (7-methoxycoumarin-4-yl)acetyl-QKLVFFAEDVK(2,4-dinitrophenyl)-OH is below 1 nmol/min*mg | Homo sapiens |
E111Q | specific activity with the substrates (7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH and (7-methoxycoumarin-4-yl)acetyl-QKLVFFAEDVK(2,4-dinitrophenyl)-OH is below 1 nmol/min*mg | Homo sapiens |
Y831A | specific activity with the substrates (7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH and (7-methoxycoumarin-4-yl)acetyl-QKLVFFAEDVK(2,4-dinitrophenyl)-OH is below 1 nmol/min*mg | Homo sapiens |
Y831D | specific activity with the substrates (7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH and (7-methoxycoumarin-4-yl)acetyl-QKLVFFAEDVK(2,4-dinitrophenyl)-OH is below 1 nmol/min*mg | Homo sapiens |
Y831F | specific activity with the substrates (7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH and (7-methoxycoumarin-4-yl)acetyl-QKLVFFAEDVK(2,4-dinitrophenyl)-OH is below 1 nmol/min*mg | Homo sapiens |
Y831K | specific activity with the substrates (7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH and (7-methoxycoumarin-4-yl)acetyl-QKLVFFAEDVK(2,4-dinitrophenyl)-OH is below 1 nmol/min*mg | Homo sapiens |
Y831N | specific activity with the substrates (7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH and (7-methoxycoumarin-4-yl)acetyl-QKLVFFAEDVK(2,4-dinitrophenyl)-OH is below 1 nmol/min*mg | Homo sapiens |
Y831W | specific activity with the substrates (7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH and (7-methoxycoumarin-4-yl)acetyl-QKLVFFAEDVK(2,4-dinitrophenyl)-OH is below 1 nmol/min*mg | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | zinc metalloendopeptidase | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
110000 | - |
- |
Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P14735 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(7-methoxycoumarin-4-yl)acetyl-QKLVFFAEDVK(2,4-dinitrophenyl)-OH + H2O | - |
Homo sapiens | ? | - |
? | |
(7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH + H2O | - |
Homo sapiens | ? | - |
? | |
amyloid beta + H2O | - |
Homo sapiens | ? | - |
? | |
insulin + H2O | - |
Homo sapiens | ? | - |
? | |
additional information | a functional requirement for active site residues F115, A140, F141, Y150, W199, F202, F820, and Y831 is established, and specific contributions of residue charge, size, and hydrophobicity to substrate binding, specificity, and proteolysis are demonstrated | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
IDE | - |
Homo sapiens |
Insulin-degrading enzyme | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.088 | - |
(7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH | pH 7.3, 37°C, mutant enzyme W199K | Homo sapiens | |
0.095 | - |
(7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH | pH 7.3, 37°C, mutant enzyme Y150K | Homo sapiens | |
0.097 | - |
(7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH | pH 7.3, 37°C, mutant enzyme F202D | Homo sapiens | |
0.097 | - |
(7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH | pH 7.3, 37°C, mutant enzyme F202K | Homo sapiens | |
0.1 | - |
(7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH | pH 7.3, 37°C, mutant enzyme F141W | Homo sapiens | |
0.108 | - |
(7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH | pH 7.3, 37°C, mutant enzyme F820Y | Homo sapiens | |
0.123 | - |
(7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH | pH 7.3, 37°C, mutant enzyme F141N | Homo sapiens | |
0.143 | - |
(7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH | pH 7.3, 37°C, mutant enzyme Y150W | Homo sapiens | |
0.148 | - |
(7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH | pH 7.3, 37°C, mutant enzyme F202W | Homo sapiens | |
0.16 | - |
(7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH | pH 7.3, 37°C, mutant enzyme F820W | Homo sapiens | |
0.16 | - |
(7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH | pH 7.3, 37°C, mutant enzyme W199Y | Homo sapiens | |
0.17 | - |
(7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH | pH 7.3, 37°C, mutant enzyme F202N | Homo sapiens | |
0.177 | - |
(7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH | pH 7.3, 37°C, mutant enzyme W199N | Homo sapiens | |
0.178 | - |
(7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH | pH 7.3, 37°C, mutant enzyme F141Y | Homo sapiens | |
0.18 | - |
(7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH | pH 7.3, 37°C, mutant enzyme W199A | Homo sapiens | |
0.198 | - |
(7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH | pH 7.3, 37°C, wild-type enzyme | Homo sapiens | |
0.2 | - |
(7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH | pH 7.3, 37°C, mutant enzyme F115Y | Homo sapiens | |
0.22 | - |
(7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH | pH 7.3, 37°C, mutant enzyme F141A | Homo sapiens | |
0.22 | - |
(7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH | pH 7.3, 37°C, mutant enzyme Y150F | Homo sapiens | |
0.24 | - |
(7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH | pH 7.3, 37°C, mutant enzyme F202Y | Homo sapiens | |
0.24 | - |
(7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH | pH 7.3, 37°C, mutant enzyme W199F | Homo sapiens | |
0.29 | - |
(7-methoxycoumarin-4-yl)acetyl-QKLVFFAEDVK(2,4-dinitrophenyl)-OH | pH 7.3, 37°C, mutant enzyme F820Y | Homo sapiens | |
0.298 | - |
(7-methoxycoumarin-4-yl)acetyl-QKLVFFAEDVK(2,4-dinitrophenyl)-OH | pH 7.3, 37°C, mutant enzyme Y150K | Homo sapiens | |
0.33 | - |
(7-methoxycoumarin-4-yl)acetyl-QKLVFFAEDVK(2,4-dinitrophenyl)-OH | pH 7.3, 37°C, mutant enzyme 141A | Homo sapiens | |
0.338 | - |
(7-methoxycoumarin-4-yl)acetyl-QKLVFFAEDVK(2,4-dinitrophenyl)-OH | pH 7.3, 37°C, mutant enzyme Y150F | Homo sapiens | |
0.45 | - |
(7-methoxycoumarin-4-yl)acetyl-QKLVFFAEDVK(2,4-dinitrophenyl)-OH | pH 7.3, 37°C, mutant enzyme Y150W | Homo sapiens | |
0.53 | - |
(7-methoxycoumarin-4-yl)acetyl-QKLVFFAEDVK(2,4-dinitrophenyl)-OH | pH 7.3, 37°C, mutant enzyme F202D | Homo sapiens | |
0.57 | - |
(7-methoxycoumarin-4-yl)acetyl-QKLVFFAEDVK(2,4-dinitrophenyl)-OH | pH 7.3, 37°C, mutant enzyme W199Y | Homo sapiens | |
0.57 | - |
(7-methoxycoumarin-4-yl)acetyl-QKLVFFAEDVK(2,4-dinitrophenyl)-OH | pH 7.3, 37°C, wild-type enzyme | Homo sapiens | |
0.625 | - |
(7-methoxycoumarin-4-yl)acetyl-QKLVFFAEDVK(2,4-dinitrophenyl)-OH | pH 7.3, 37°C, mutant enzyme 141W | Homo sapiens | |
0.72 | - |
(7-methoxycoumarin-4-yl)acetyl-QKLVFFAEDVK(2,4-dinitrophenyl)-OH | pH 7.3, 37°C, mutant enzyme F115Y | Homo sapiens | |
0.725 | - |
(7-methoxycoumarin-4-yl)acetyl-QKLVFFAEDVK(2,4-dinitrophenyl)-OH | pH 7.3, 37°C, mutant enzyme F115A | Homo sapiens | |
0.73 | - |
(7-methoxycoumarin-4-yl)acetyl-QKLVFFAEDVK(2,4-dinitrophenyl)-OH | pH 7.3, 37°C, mutant enzyme F115W | Homo sapiens | |
0.735 | - |
(7-methoxycoumarin-4-yl)acetyl-QKLVFFAEDVK(2,4-dinitrophenyl)-OH | pH 7.3, 37°C, mutant enzyme F202Y | Homo sapiens | |
0.78 | - |
(7-methoxycoumarin-4-yl)acetyl-QKLVFFAEDVK(2,4-dinitrophenyl)-OH | pH 7.3, 37°C, mutant enzyme 141Y | Homo sapiens | |
0.803 | - |
(7-methoxycoumarin-4-yl)acetyl-QKLVFFAEDVK(2,4-dinitrophenyl)-OH | pH 7.3, 37°C, mutant enzyme F202W | Homo sapiens | |
0.85 | - |
(7-methoxycoumarin-4-yl)acetyl-QKLVFFAEDVK(2,4-dinitrophenyl)-OH | pH 7.3, 37°C, mutant enzyme F820W | Homo sapiens | |
1.1 | - |
(7-methoxycoumarin-4-yl)acetyl-QKLVFFAEDVK(2,4-dinitrophenyl)-OH | pH 7.3, 37°C, mutant enzyme Y150F | Homo sapiens |