Literature summary for 3.4.24.56 extracted from

Sharma, S.K.; Chorell, E.; Wittung-Stafshede, P.
Insulin-degrading enzyme is activated by the C-terminus of alpha-synuclein (2015), Biochem. Biophys. Res. Commun., 466, 192-195 .

Search for more literature for 3.4.24.56

Activating Compound

Activating Compound	Comment	Organism	Structure
alpha-synuclein	a peptide with the C-terminal 44 residues of alpha-synuclein increases insulin-degrading enzyme proteolysis to the same degree as full-length alpha-synuclein. A peptide containing the first 97 residues of alpha-synuclein does not improve insulin-degrading enzyme activity. Because the alpha-synuclein C-terminus is acidic, the interaction appears to involve electrostatic attraction with basic exosite of insulin-degrading enzyme	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0099	-	(7-methoxycoumarin-4-yl)acetyl-RPPGFSAFK(2,4-dinitrophenyl)-OH	37°C, pH not specified in the publication	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P14735	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(7-methoxycoumarin-4-yl)acetyl-RPPGFSAFK(2,4-dinitrophenyl)-OH + H2O	-	Homo sapiens	?	-	?

Synonyms

Synonyms	Comment	Organism
Insulin-degrading enzyme	-	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.23	-	(7-methoxycoumarin-4-yl)acetyl-RPPGFSAFK(2,4-dinitrophenyl)-OH	37°C, pH not specified in the publication	Homo sapiens

General Information

General Information	Comment	Organism
metabolism	the enzyme plays a key role in type-2 diabetes	Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
23.4	-	(7-methoxycoumarin-4-yl)acetyl-RPPGFSAFK(2,4-dinitrophenyl)-OH	37°C, pH not specified in the publication	Homo sapiens

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Release 2023.1 (January 2023)