Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.24.39 extracted from

  • Maeda, H.; Katase, T.; Sakai, D.; Takeuchi, M.; Kusumoto, K.; Amano, H.; Ishida, H.; Abe, K.; Yamagata, Y.
    A novel non-thermostable deuterolysin from Aspergillus oryzae (2016), Biosci. Biotechnol. Biochem., 80, 1813-1819 .
    View publication on PubMed
Search for more literature for 3.4.24.39

Cloned(Commentary)

Cloned (Comment) Organism
overexpressing in Aspergillus oryzae niaD300 Aspergillus oryzae

Organism

Organism UniProt Comment Textmining
Aspergillus oryzae P46076
-
-
Aspergillus oryzae Q2UP30
-
-
Aspergillus oryzae ATCC 42149 P46076
-
-
Aspergillus oryzae ATCC 42149 Q2UP30
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Aspergillus oryzae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-casein + H2O degradation with low specific activity Aspergillus oryzae ?
-
 ?
benzyloxycarbonyl-Val-Lys-Met-7-amido-4-methylcoumarin + H2O second-best substrate Aspergillus oryzae ?
-
 ?
beta-lactoglobulin + H2O degradation with low specific activity Aspergillus oryzae ?
-
 ?
Boc-Arg-Val-Arg-Arg-7-amido-4-methylcoumarin + H2O most favorable substrate Aspergillus oryzae ?
-
 ?
Boc-Val-Leu-Lys-7-amido-4-methylcoumarin + H2O
-
 Aspergillus oryzae ?
-
 ?
Boc-Val-Leu-Lys-7-amido-4-methylcoumarin + H2O degradation with low specific activity Aspergillus oryzae ?
-
 ?
casein + H2O degradation with low specific activity Aspergillus oryzae ?
-
 ?
casein + H2O degradation with low specific activity Aspergillus oryzae ATCC 42149 ?
-
 ?
clupeine + H2O degradation with high specific activity Aspergillus oryzae ?
-
 ?
Elastin + H2O
-
 Aspergillus oryzae ?
-
 ?
Elastin + H2O
-
 Aspergillus oryzae ATCC 42149 ?
-
 ?
Fibrin + H2O degradation with low specific activity Aspergillus oryzae ?
-
 ?
Fibrin + H2O degradation with low specific activity Aspergillus oryzae ATCC 42149 ?
-
 ?
Glt-Ala-Ala-Phe-7-amido-4-methylcoumarin + H2O
-
 Aspergillus oryzae ?
-
 ?
Glt-Ala-Ala-Phe-7-amido-4-methylcoumarin + H2O degradation with low specific activity Aspergillus oryzae ?
-
 ?
histone + H2O degradation with high specific activity Aspergillus oryzae ?
-
 ?
histone + H2O degradation with high specific activity Aspergillus oryzae ATCC 42149 ?
-
 ?
additional information DeuA can not digest elastin and collagen. No hydrolysis of benzyloxycarbonyl-Val-Lys-Met-7-amido-4-methylcoumarin, benzyloxycarbonyl-Leu-Arg-7-amido-4-methylcoumarin, benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin and Suc-Gly-Pro-7-amido-4-methylcoumarin. DeuA and DeuB have similar digestion property against the protein substrates, but DeuB has a wider substrate specificity compared with DeuA Aspergillus oryzae ?
-
 ?
additional information DeuB can not digest collagen. DeuA and DeuB have similar digestion property against the protein substrates, but DeuB has a wider substrate specificity compared with DeuA Aspergillus oryzae ?
-
 ?
additional information DeuA can not digest elastin and collagen. No hydrolysis of benzyloxycarbonyl-Val-Lys-Met-7-amido-4-methylcoumarin, benzyloxycarbonyl-Leu-Arg-7-amido-4-methylcoumarin, benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin and Suc-Gly-Pro-7-amido-4-methylcoumarin. DeuA and DeuB have similar digestion property against the protein substrates, but DeuB has a wider substrate specificity compared with DeuA Aspergillus oryzae ATCC 42149 ?
-
 ?
additional information DeuB can not digest collagen. DeuA and DeuB have similar digestion property against the protein substrates, but DeuB has a wider substrate specificity compared with DeuA Aspergillus oryzae ATCC 42149 ?
-
 ?
ovalbumin + H2O degradation with low specific activity Aspergillus oryzae ?
-
 ?
ovalbumin + H2O degradation with low specific activity Aspergillus oryzae ATCC 42149 ?
-
 ?
salmine + H2O degradation with high specific activity Aspergillus oryzae ?
-
 ?
Suc-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O
-
 Aspergillus oryzae ?
-
 ?
Suc-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O degradation with low specific activity Aspergillus oryzae ?
-
 ?

Synonyms

Synonyms Comment Organism
DeuA
-
 Aspergillus oryzae
deuB
-
 Aspergillus oryzae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50 60
-
 Aspergillus oryzae
50 70
-
 Aspergillus oryzae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 pH 7.0, 10 min, 15% loss of activity Aspergillus oryzae
30
-
 pH 7.0, 10 min, no loss of activity Aspergillus oryzae
40
-
 pH 7.0, 10 min, 10% loss of activity Aspergillus oryzae
40
-
 pH 7.0, 10 min, no loss of activity Aspergillus oryzae
50
-
 pH 7.0, 10 min, 50% loss of activity Aspergillus oryzae
50
-
 pH 7.0, 10 min, 5% loss of activity Aspergillus oryzae
60
-
 pH 7.0, 10 min, 50% loss of activity Aspergillus oryzae
60
-
 pH 7.0, 10 min, 10% loss of activity Aspergillus oryzae
70
-
 pH 7.0, 10 min, 85% loss of activity Aspergillus oryzae
70
-
 pH 7.0, 10 min, 30% loss of activity Aspergillus oryzae
80
-
 pH 7.0, 10 min, 35% loss of activity Aspergillus oryzae
80
-
 pH 7.0, 10 min, DeuB completely loses its activity Aspergillus oryzae
90
-
 pH 7.0, 10 min, 30% loss of activity Aspergillus oryzae
100
-
 pH 7.0, 10 min, 15% loss of activity Aspergillus oryzae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 10
-
 Aspergillus oryzae

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 11 stable Aspergillus oryzae

Expression

Organism Comment Expression
Aspergillus oryzae transcription of deuB is observed at an early stage of CDN liquid culture (24 h) and the prolonged cultivation reduces the transcription of deuB. Addition of NaNO3 inhibits the upregulation of deuB transcription down
Aspergillus oryzae proteinous nitrogen sources such as skimmed milk and gelatin instead of NaNO3 upregulate the transcription level of deuB. Addition of NaNO3 inhibits the upregulation of deuB transcription up