KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0119 | - |
Pyr-Arg-Thr-Lys-Arg-4-methylcoumarin-7-amide | pH 9.0, 30°C, Co-substituted deuterolysin | Aspergillus oryzae | |
0.0237 | - |
Pyr-Arg-Thr-Lys-Arg-4-methylcoumarin-7-amide | pH 9.0, 30°C | Aspergillus oryzae | |
0.0264 | - |
N-butoxycarbonyl-arginyl-valyl-arginyl-arginyl-4-methylcoumarin-7-amide | pH 9.0, 30°C | Aspergillus oryzae | |
0.0275 | - |
N-butoxycarbonyl-arginyl-valyl-arginyl-arginyl-4-methylcoumarin-7-amide | pH 9.0, 30°C, Co-substituted deuterolysin | Aspergillus oryzae | |
0.0473 | - |
Z-Arg-Arg-4-methylcoumarin-7-amide | pH 9.0, 30°C | Aspergillus oryzae | |
0.0522 | - |
Z-Arg-Arg-4-methylcoumarin-7-amide | pH 9.0, 30°C, Co-substituted deuterolysin | Aspergillus oryzae | |
0.0638 | - |
N-butoxycarbonyl-Leu-Arg-Arg-4-methylcoumarin-7-amide | pH 9.0, 30°C, Co-substituted deuterolysin | Aspergillus oryzae | |
0.07 | - |
N-butoxycarbonyl-Leu-Arg-Arg-4-methylcoumarin-7-amide | pH 9.0, 30°C | Aspergillus oryzae | |
0.146 | - |
N-butoxycarbonyl-Leu-Lys-Arg-4-methylcoumarin-7-amide | pH 9.0, 30°C | Aspergillus oryzae | |
0.1573 | - |
N-butoxycarbonyl-Leu-Lys-Arg-4-methylcoumarin-7-amide | pH 9.0, 30°C, Co-substituted deuterolysin | Aspergillus oryzae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | zinc-protease, contains one mole of zinc per mole of enzyme | Aspergillus oryzae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
19000 | - |
- |
Aspergillus oryzae |
19020 | - |
amino acid sequence | Aspergillus oryzae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus oryzae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Aspergillus oryzae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
calf thymus histone 4 + H2O | - |
Aspergillus oryzae | ? | - |
? | |
additional information | almost no hydrolysis toward mono or certain dibasic peptide 4-methylcoumarin-7-amides like N-butoxycarbonyl-Leu-Ser-Thr-Arg-4-methylcoumarin-7-amide, N-butoxycarbonyl-Ile-Glu-Gly-Arg-4-methylcoumarin-7-amide, N-butoxycarbonyl-Ala-Gly-Pro-Arg-4-methylcoumarin-7-amide, N-butoxycarbonyl-Gln-Gly-Arg-4-methylcoumarin-7-amide, N-butoxycarbonyl-Leu-Gly-Arg-4-methylcoumarin-7-amide, N-butoxycarbonyl-Leu-Thr-Arg-4-methylcoumarin-7-amide, Z-Phe-Arg-4-methylcoumarin-7-amide, Arg-4-methylcoumarin-7-amide, Lys-4-methylcoumarin-7-amide, N-butoxycarbonyl-Gly-Arg-Arg-4-methylcoumarin-7-amide, N-butoxycarbonyl-Gln-Arg-Arg-4-methylcoumarin-7-amide, and N-butoxycarbonyl-Gln-Lys-Lys-4-methylcoumarin-7-amide | Aspergillus oryzae | ? | - |
? | |
N-butoxycarbonyl-arginyl-valyl-arginyl-arginyl-4-methylcoumarin-7-amide + H2O | - |
Aspergillus oryzae | ? | - |
? | |
N-butoxycarbonyl-Leu-Arg-Arg-4-methylcoumarin-7-amide + H2O | - |
Aspergillus oryzae | ? | - |
? | |
N-butoxycarbonyl-Leu-Lys-Arg-4-methylcoumarin-7-amide + H2O | - |
Aspergillus oryzae | ? | - |
? | |
Pyr-Arg-Thr-Lys-Arg-4-methylcoumarin-7-amide + H2O | - |
Aspergillus oryzae | ? | - |
? | |
Z-Arg-Arg-4-methylcoumarin-7-amide + H2O | - |
Aspergillus oryzae | ? | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 100 | extremely stable at 100°C, but unstable near 75°C because of self-digestion | Aspergillus oryzae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00065 | - |
Z-Arg-Arg-4-methylcoumarin-7-amide | pH 9.0, 30°C, Co-substituted deuterolysin | Aspergillus oryzae | |
0.00082 | - |
Pyr-Arg-Thr-Lys-Arg-4-methylcoumarin-7-amide | pH 9.0, 30°C, Co-substituted deuterolysin | Aspergillus oryzae | |
0.0009 | - |
Z-Arg-Arg-4-methylcoumarin-7-amide | pH 9.0, 30°C | Aspergillus oryzae | |
0.0017 | - |
N-butoxycarbonyl-Leu-Arg-Arg-4-methylcoumarin-7-amide | pH 9.0, 30°C, Co-substituted deuterolysin | Aspergillus oryzae | |
0.0022 | - |
Pyr-Arg-Thr-Lys-Arg-4-methylcoumarin-7-amide | pH 9.0, 30°C | Aspergillus oryzae | |
0.0035 | - |
N-butoxycarbonyl-Leu-Lys-Arg-4-methylcoumarin-7-amide | pH 9.0, 30°C, Co-substituted deuterolysin | Aspergillus oryzae | |
0.0045 | - |
N-butoxycarbonyl-Leu-Arg-Arg-4-methylcoumarin-7-amide | pH 9.0, 30°C | Aspergillus oryzae | |
0.009 | - |
N-butoxycarbonyl-Leu-Lys-Arg-4-methylcoumarin-7-amide | pH 9.0, 30°C | Aspergillus oryzae | |
0.017 | - |
N-butoxycarbonyl-arginyl-valyl-arginyl-arginyl-4-methylcoumarin-7-amide | pH 9.0, 30°C, Co-substituted deuterolysin | Aspergillus oryzae | |
0.022 | - |
N-butoxycarbonyl-arginyl-valyl-arginyl-arginyl-4-methylcoumarin-7-amide | pH 9.0, 30°C | Aspergillus oryzae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Aspergillus oryzae |