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Literature summary for 3.4.24.29 extracted from
- Staphylococcus aureus metalloprotease aureolysin cleaves complement C3 to mediate immune evasion (2011), J. Immunol., 186, 6445-6453.
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | the enzyme is secreted | Staphylococcus aureus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | metalloprotease | Staphylococcus aureus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| complement component C3 + H2O | Staphylococcus aureus | aureolysin cleaves purified C3 specifically in the alpha-chain, close to the C3 convertase cleavage site, yielding active C3a and C3b. In serum, the aureolysin-generated C3b is further degraded by host factors | C3a+SN + C3b2SN | both products are active | ? |  |
| complement component C3 + H2O | Staphylococcus aureus KV27 | aureolysin cleaves purified C3 specifically in the alpha-chain, close to the C3 convertase cleavage site, yielding active C3a and C3b. In serum, the aureolysin-generated C3b is further degraded by host factors | C3a+SN + C3b2SN | both products are active | ? | |
| additional information | Staphylococcus aureus | Aureolysin collaborates with host factors to inactivate C3b | ? | - |
? | |
| additional information | Staphylococcus aureus KV27 | Aureolysin collaborates with host factors to inactivate C3b | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Staphylococcus aureus | - |
a clinical isolate | - |
| Staphylococcus aureus KV27 | - |
a clinical isolate | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| complement component C3 + H2O | aureolysin cleaves purified C3 specifically in the alpha-chain, close to the C3 convertase cleavage site, yielding active C3a and C3b. In serum, the aureolysin-generated C3b is further degraded by host factors | Staphylococcus aureus | C3a+SN + C3b2SN | both products are active | ? | |
| complement component C3 + H2O | aureolysin cleaves purified C3 specifically in the alpha-chain, close to the C3 convertase cleavage site, yielding active C3a and C3b | Staphylococcus aureus | C3a+SN + C3b2SN | both products are active | ? | |
| complement component C3 + H2O | aureolysin cleaves purified C3 specifically in the alpha-chain, close to the C3 convertase cleavage site, yielding active C3a and C3b. In serum, the aureolysin-generated C3b is further degraded by host factors | Staphylococcus aureus KV27 | C3a+SN + C3b2SN | both products are active | ? | |
| complement component C3 + H2O | aureolysin cleaves purified C3 specifically in the alpha-chain, close to the C3 convertase cleavage site, yielding active C3a and C3b | Staphylococcus aureus KV27 | C3a+SN + C3b2SN | both products are active | ? | |
| additional information | Aureolysin collaborates with host factors to inactivate C3b | Staphylococcus aureus | ? | - |
? | |
| additional information | Aureolysin collaborates with host factors to inactivate C3b | Staphylococcus aureus KV27 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| C3 convertase | - |
Staphylococcus aureus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Staphylococcus aureus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
20 min, purified enzyme, inactivation | Staphylococcus aureus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Staphylococcus aureus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | Staphylococcus aureus metalloprotease aureolysin cleaves complement C3 to mediate immune evasion and is a potent complement inhibitor dependent on its proteolytic activity. Aureolysin effectively inhibits phagocytosis and killing of bacteria by neutrophils in the human host, e.g. in U937 cells, aureolysin inhibits C3b deposition and C5a generation, overview. Aureolysin is essential and sufficient for C3 cleavage by bacterial supernatant, but acts in synergy with host regulators to inactivate C3 thereby effectively dampening the host immune response. Aureolysin acts as a C3 convertase | Staphylococcus aureus |
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