Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | microwave power output increases the level of activity of the immobilized thermolysin by 4.5fold, optimally at 40 W, overview | Bacillus thermoproteolyticus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | evaluation of an efficient method for the immobilization of thermolysin using sodium chloride salting-in and consecutive microwave irradiation, overview. 4.6% of the relative activity for the immobilized thermolysin is detected when the immobilization mixture contains no salts, including ZnCl2, CaCl2 or NaCl | Bacillus thermoproteolyticus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | CaCl2 does not exert any significant influence on the activity of immobilized thermolysin, but stabilizes the enzyme in absence of Zn2+ | Bacillus thermoproteolyticus | |
NaCl | activates at 3 M | Bacillus thermoproteolyticus | |
Zn2+ | a zinc metalloprotease | Bacillus thermoproteolyticus |
Organic Solvent | Comment | Organism |
---|---|---|
2-methyl-2-butanol | no reduction in activity of immobilized thermolysin after 2 h in 5% tert-amyl alcohol, whereas the free enzyme loses 32.5% of its catalytic activity | Bacillus thermoproteolyticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus thermoproteolyticus | - |
- |
- |
Bacillus thermoproteolyticus Rokko | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | - |
Bacillus thermoproteolyticus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
casein + H2O | - |
Bacillus thermoproteolyticus | ? | - |
? | |
casein + H2O | - |
Bacillus thermoproteolyticus Rokko | ? | - |
? | |
N-carbobenzoxy-L-Asp-L-Phe methyl ester + H2O | - |
Bacillus thermoproteolyticus | ? | - |
? | |
N-carbobenzoxy-L-Asp-L-Phe methyl ester + H2O | - |
Bacillus thermoproteolyticus Rokko | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Neutral metalloproteinase | - |
Bacillus thermoproteolyticus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Bacillus thermoproteolyticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Bacillus thermoproteolyticus |