Cloned (Comment) | Organism |
---|---|
cloned in Bacillus subtilis DB117, plasmids subcloned in Escherichia coli | Bacillus thermoproteolyticus |
Protein Variants | Comment | Organism |
---|---|---|
D226A | site-directed mutagenesis | Bacillus thermoproteolyticus |
Y157F | site-directed mutagenesis | Bacillus thermoproteolyticus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
HACBO-Gly | - |
Bacillus thermoproteolyticus | |
phosphoramidon | - |
Bacillus thermoproteolyticus | |
thiorphan | - |
Bacillus thermoproteolyticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.21 | - |
leucine enkephalin | pH 7.4, 37°C, wild-type | Bacillus thermoproteolyticus | |
0.48 | - |
leucine enkephalin | pH 7.4, 37°C, mutant Y157F | Bacillus thermoproteolyticus | |
1.985 | - |
leucine enkephalin | pH 7.4, 37°C, mutant D226A | Bacillus thermoproteolyticus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
Western blot | Bacillus thermoproteolyticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus thermoproteolyticus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutated thermolysin | Bacillus thermoproteolyticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
leucine enkephalin + H2O | - |
Bacillus thermoproteolyticus | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.94 | - |
leucine enkephalin | pH 7.4, 37°C, mutant Y157F | Bacillus thermoproteolyticus | |
155.5 | - |
leucine enkephalin | pH 7.4, 37°C, wild-type | Bacillus thermoproteolyticus | |
156 | - |
leucine enkephalin | pH 7.4, 37°C, wild-type | Bacillus thermoproteolyticus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000031 | - |
phosphoramidon | pH 7.4, 37°C, mutant Y157F | Bacillus thermoproteolyticus | |
0.00006 | - |
phosphoramidon | pH 7.4, 37°C, wild-type | Bacillus thermoproteolyticus | |
0.00019 | - |
phosphoramidon | pH 7.4, 37°C, mutant D226A | Bacillus thermoproteolyticus | |
0.0016 | - |
thiorphan | pH 7.4, 37°C, wild-type | Bacillus thermoproteolyticus | |
0.0031 | - |
HACBO-Gly | pH 7.4, 37°C, wild-type | Bacillus thermoproteolyticus | |
0.0076 | - |
thiorphan | pH 7.4, 37°C, mutant Y157F | Bacillus thermoproteolyticus | |
0.013 | - |
thiorphan | pH 7.4, 37°C, mutant D226A | Bacillus thermoproteolyticus | |
0.019 | - |
HACBO-Gly | pH 7.4, 37°C, mutant D226A | Bacillus thermoproteolyticus | |
0.031 | - |
HACBO-Gly | pH 7.4, 37°C, mutant Y157F | Bacillus thermoproteolyticus |