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Literature summary for 3.4.24.27 extracted from

  • Marie-Claire, C.; Ruffet, E.; Tiraboschi, G.; Fournie-Zaluski, M.C.
    Differences in transition state stabilization between thermolysin (EC 3.4.24.27) and neprilysin (EC 3.4.24.11) (1998), FEBS Lett., 438, 215-219.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
cloned in Bacillus subtilis DB117, plasmids subcloned in Escherichia coli Bacillus thermoproteolyticus

Protein Variants

Protein Variants Comment Organism
D226A site-directed mutagenesis Bacillus thermoproteolyticus
Y157F site-directed mutagenesis Bacillus thermoproteolyticus

Inhibitors

Inhibitors Comment Organism Structure
HACBO-Gly
-
Bacillus thermoproteolyticus
phosphoramidon
-
Bacillus thermoproteolyticus
thiorphan
-
Bacillus thermoproteolyticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.21
-
leucine enkephalin pH 7.4, 37°C, wild-type Bacillus thermoproteolyticus
0.48
-
leucine enkephalin pH 7.4, 37°C, mutant Y157F Bacillus thermoproteolyticus
1.985
-
leucine enkephalin pH 7.4, 37°C, mutant D226A Bacillus thermoproteolyticus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
Western blot Bacillus thermoproteolyticus

Organism

Organism UniProt Comment Textmining
Bacillus thermoproteolyticus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and mutated thermolysin Bacillus thermoproteolyticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
leucine enkephalin + H2O
-
Bacillus thermoproteolyticus ?
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.94
-
leucine enkephalin pH 7.4, 37°C, mutant Y157F Bacillus thermoproteolyticus
155.5
-
leucine enkephalin pH 7.4, 37°C, wild-type Bacillus thermoproteolyticus
156
-
leucine enkephalin pH 7.4, 37°C, wild-type Bacillus thermoproteolyticus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000031
-
phosphoramidon pH 7.4, 37°C, mutant Y157F Bacillus thermoproteolyticus
0.00006
-
phosphoramidon pH 7.4, 37°C, wild-type Bacillus thermoproteolyticus
0.00019
-
phosphoramidon pH 7.4, 37°C, mutant D226A Bacillus thermoproteolyticus
0.0016
-
thiorphan pH 7.4, 37°C, wild-type Bacillus thermoproteolyticus
0.0031
-
HACBO-Gly pH 7.4, 37°C, wild-type Bacillus thermoproteolyticus
0.0076
-
thiorphan pH 7.4, 37°C, mutant Y157F Bacillus thermoproteolyticus
0.013
-
thiorphan pH 7.4, 37°C, mutant D226A Bacillus thermoproteolyticus
0.019
-
HACBO-Gly pH 7.4, 37°C, mutant D226A Bacillus thermoproteolyticus
0.031
-
HACBO-Gly pH 7.4, 37°C, mutant Y157F Bacillus thermoproteolyticus