Cloned (Comment) | Organism |
---|---|
cloned in Bacillus subtilis DB117, plasmids subcloned in Escherichia coli | Bacillus thermoproteolyticus |
Crystallization (Comment) | Organism |
---|---|
- |
Bacillus thermoproteolyticus |
Protein Variants | Comment | Organism |
---|---|---|
D170A | site-directed mutagenesis | Bacillus thermoproteolyticus |
R203M | site-directed mutagenesis | Bacillus thermoproteolyticus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
phosphoramidon | - |
Bacillus thermoproteolyticus | |
Retrothiorphan | - |
Bacillus thermoproteolyticus | |
thiorphan | - |
Bacillus thermoproteolyticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
leucine enkephalin | pH 6.8, 37°C, wild-type | Bacillus thermoproteolyticus | |
0.24 | - |
leucine enkephalin | pH 6.8, 37°C, mutant D170A | Bacillus thermoproteolyticus | |
0.24 | - |
leucine enkephalin | pH 6.8, 37°C, mutant R203M | Bacillus thermoproteolyticus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | contains 4 structural calcium ions | Bacillus thermoproteolyticus | |
Zn2+ | zinc-metallopeptidase | Bacillus thermoproteolyticus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
Western blot | Bacillus thermoproteolyticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus thermoproteolyticus | - |
Rokko | - |
Bacillus thermoproteolyticus Rokko | - |
Rokko | - |
Purification (Comment) | Organism |
---|---|
wild-type and mutated thermolysin | Bacillus thermoproteolyticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
leucine enkephalin + H2O | - |
Bacillus thermoproteolyticus | ? | - |
? | |
leucine enkephalin + H2O | - |
Bacillus thermoproteolyticus Rokko | ? | - |
? | |
[3H]leucine enkephalin + H2O | - |
Bacillus thermoproteolyticus | ? | - |
? | |
[3H]leucine enkephalin + H2O | - |
Bacillus thermoproteolyticus Rokko | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.083 | - |
leucine enkephalin | pH 6.8, 37°C, mutant R203M | Bacillus thermoproteolyticus | |
2.94 | - |
leucine enkephalin | pH 6.8, 37°C, mutant D170A | Bacillus thermoproteolyticus | |
1555 | - |
leucine enkephalin | pH 6.8, 37°C, wild-type | Bacillus thermoproteolyticus | |
1560 | - |
leucine enkephalin | pH 6.8, 37°C, wild-type | Bacillus thermoproteolyticus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00006 | - |
phosphoramidon | pH 6.8, 37°C, wild-type | Bacillus thermoproteolyticus | |
0.0008 | - |
Retrothiorphan | pH 6.8, 37°C, wild-type | Bacillus thermoproteolyticus | |
0.0016 | - |
thiorphan | pH 6.8, 37°C, wild-type | Bacillus thermoproteolyticus | |
0.0033 | - |
thiorphan | pH 6.8, 37°C, mutant D170A | Bacillus thermoproteolyticus | |
0.0047 | - |
phosphoramidon | pH 6.8, 37°C, mutant R203M | Bacillus thermoproteolyticus | |
0.16 | - |
thiorphan | pH 6.8, 37°C, mutant R203M | Bacillus thermoproteolyticus | |
0.16 | - |
Retrothiorphan | pH 6.8, 37°C, mutant R203M | Bacillus thermoproteolyticus |