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Literature summary for 3.4.24.14 extracted from

  • Hojima, Y.; Morgelin, M.M.; Engel, J.; Boutillon, M.M.; Van der Rest, M.; McKenzie, J.; Chen, G.C.; Rafi, N.; Romanic, A.M.; Prockop, D.J.
    Characterization of type I procollagen N-proteinase from fetal bovine tendon and skin. Purification of the 500-kilodalton form of the enzyme from bovine tendon (1994), J. Biol. Chem., 269, 11381-11390.
    View publication on PubMed
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General Stability

General Stability Organism
50% loss of activity upon lyophilization Bos taurus
Ca2+ stabilizes Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 100% inhibition at 1 mM Bos taurus
2-mercaptoethanol 26% inhibition at 1 mM Bos taurus
ammonium persulfate 94% inhibition at 10 micromol/ml Bos taurus
Co2+ 20% inhibition at 0.01 mM Bos taurus
concanavalin A 91% inhibition at 50 micromol/ml Bos taurus
Cu2+ 60% inhibition at 0.01 mM Bos taurus
EDTA 100% inhibition at 7.5 mM Bos taurus
Fetal bovine serum 34% inhibition at concentration of 2% Bos taurus
heparan sulfate 38% inhibition at 0.5 micromol/ml Bos taurus
heparin 52-58% inhibition at 0.05 micromol/ml Bos taurus
Ni2+ 19% inhibition at 0.01 mM Bos taurus
phosphate ,46% inhibition at 2 mM Bos taurus
Poly-L-aspartate 24% inhibition at 50 micromol/ml Bos taurus
Poly-L-glutamate 40% inhibition at 50 micromol/ml Bos taurus
SDS 79% inhibition at 50 micromol/ml Bos taurus
Zn2+ 73% inhibition at 0.01 mM Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000035
-
 procollagen I
-
 Bos taurus

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ increase of activity Bos taurus
Ca2+ required for maximal activity Bos taurus
Mg2+ increase of activity Bos taurus
Mn2+ increase of activity Bos taurus
Sr2+ increase of activity Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
500000
-
 gel filtration Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
procollagen I + H2O Bos taurus
-
 ?
-
 ?
procollagen II + H2O Bos taurus
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
side-chain modification glycoprotein Bos taurus

Purification (Commentary)

Purification (Comment) Organism
from tendon Bos taurus
partially from skin Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
skin
-
 Bos taurus
-
tendon
-
 Bos taurus
-

Storage Stability

Storage Stability Organism
25°C, 2 days, stable Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
procollagen I + H2O not procollagen III Bos taurus pCCollagen I + oligopeptide partially processed procollagen containing only the C-propeptides ?
procollagen I + H2O
-
 Bos taurus ?
-
 ?
procollagen II + H2O
-
 Bos taurus pCCollagen II + oligopeptide partially processed procollagen containing only the C-propeptides ?
procollagen II + H2O
-
 Bos taurus ?
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 90% of activity recovered after 60 min Bos taurus
55
-
 10% of activity recovered after 60 min Bos taurus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.08
-
 procollagen I
-
 Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.3
-
 procollagen I Bos taurus

pH Range

pH Minimum pH Maximum Comment Organism
5.5
-
 inactive below, procollagen I as substrate Bos taurus
6.7 9.5 procollagen I as substrate, 45% of optimum activity at pH 6.7 and pH 9.5 Bos taurus

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.7 9.6
-
 Bos taurus