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Literature summary for 3.4.23.B8 extracted from

  • Ding, Y.S.; Owen, S.M.; Lal, R.B.; Ikeda, R.A.
    Efficient expression and rapid purification of human T-cell leukemia virus type 1 protease (1998), J. Virol., 72, 3383-3386.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.23.B8

Cloned(Commentary)

Cloned (Comment) Organism
construction of a pET19-based expression clone containing HTLV-1 protease fused to a decahistidine-containing leader peptide, expression in Escherichia coli, autoprocessing of the purified fusion protein yields a 14000 Da protease Human T-cell leukemia virus type I

Inhibitors

Inhibitors Comment Organism Structure
pepstatin A
-
 Human T-cell leukemia virus type I

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
 APQVLPVMHPHG
-
 Human T-cell leukemia virus type I

Organism

Organism UniProt Comment Textmining
Human T-cell leukemia virus type I
-
-
-

Purification (Commentary)

Purification (Comment) Organism
pET19-based expression clone containing HTLV-1 protease fused to a decahistidine-containing leader peptide Human T-cell leukemia virus type I

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
APQVLPVMHPHG + H2O
-
 Human T-cell leukemia virus type I APQVL + PVMHPHG
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.143
-
 APQVLPVMHPHG
-
 Human T-cell leukemia virus type I

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3
-
-
 Human T-cell leukemia virus type I

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.2
-
 pepstatin A
-
 Human T-cell leukemia virus type I