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Literature summary for 3.4.23.B1 extracted from

  • Maurer, A.; Kalbacher, H.
    Pepstatin pull-down at high pH is a powerful tool for detection and analysis of napsin A (2019), Biochem. Biophys. Res. Commun., 515, 145-148 .
    View publication on PubMed
Search for more literature for 3.4.23.B1

Application

Application Comment Organism
diagnostics napsin A is an intracellular aspartic protease and biomarker of various malignancies like lung adenocarcinoma and ovarian clear cell carcinoma Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of C-terminally His-tagged enzyme in Escherichia coli strain BL21 (DE3), recombinant overexpression in HEK-293 cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
pepstatin A napsin A binds over a broad pH range to this inhibitor, highly selective binding of napsin A Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens O96009
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein deglycosylation by PNGase F Homo sapiens
proteolytic modification model of successive maturation, overview Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
recombinant C-terminally His-tagged enzyme from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
HEK-293 cell
-
 Homo sapiens
-
kidney
-
 Homo sapiens
-
lung
-
 Homo sapiens
-
lung adenocarcinoma cell
-
 Homo sapiens
-
additional information napsin A is predominantly expressed in lung and kidney. Lung napsin A in many mammals is a heterogeneous enzyme with a characteristic ladder-like appearance in SDS-PAGE that might be caused by proteolytically processed N- and/or C-termini, in contrast to the more homogeneous form found in kidneys and primary lung adenocarcinoma Homo sapiens
-
ovarian clear cell carcinoma cell
-
 Homo sapiens
-
ovary
-
 Homo sapiens
-

Synonyms

Synonyms Comment Organism
napsin A
-
 Homo sapiens

General Information

General Information Comment Organism
additional information pepstatin pull-down at high pH is a powerful tool for detection and analysis of napsin A. Method development for specific enrichment of napsin A from clinical or biological specimens, that facilitates detailed analysis of the protein, evaluation, overview Homo sapiens
physiological function napsin A is an intracellular aspartic protease and biomarker of various malignancies like lung adenocarcinoma and ovarian clear cell carcinoma Homo sapiens