Protein Variants | Comment | Organism |
---|---|---|
D16N | no processing activity | Escherichia coli |
D62M | no processing activity | Escherichia coli |
D62N | no processing activity | Escherichia coli |
H90Q | some minor processing activity | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | conserved amino-acid residues involved in cadmium ligation in the crystal are essential for the endoproteolytic activity in HycI | Escherichia coli | |
Ni2+ | the reaction requires nickel to be bound to the precursor and the protease does not have a function in nickel delivery to the substrate. Radioactive labelling of cells with 63Ni, devoid of endopeptidase, resolves several forms of the precursor which are possibly intermediates in the maturation pathway. The endopeptidase uses the metal in the large subunit of [NiFe]-hydrogenases as a recognition motif | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0AEV9 | - |
- |
Purification (Comment) | Organism |
---|---|
purified endopeptidases HycI is devoid of metal | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
precursor of the large subunit of hydrogenase 3 + H2O | HycI is specific for hydrogenase 3 maturation | Escherichia coli | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
HYCI | - |
Escherichia coli |