Cloned (Comment) | Organism |
---|---|
overexpressed without its signal sequence in Escherichia coli K-12 strain DH5alpha using a T7 system | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
G216K/K217G | recombinant ompT variant in order to abolish autoproteolysis | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diisopropylfluorophosphate | significant inhibition only at high concentrations | Escherichia coli | |
phenylmethylsulfonyl fluoride | poor inhibitor, only at high concentrations | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0003 | - |
o-aminobenzoyl-Ala-Arg-Arg-Ala-3-nitrotyrosine-NH2 | pH 6.5, 37°C, wild-type, purified from outer membrane | Escherichia coli | |
0.0004 | - |
o-aminobenzoyl-Ala-Arg-Arg-Ala-3-nitrotyrosine-NH2 | pH 6.5, 37°C, wild-type, recombinant expressed | Escherichia coli | |
0.0011 | - |
o-aminobenzoyl-Ala-Arg-Arg-Ala-3-nitrotyrosine-NH2 | pH 6.5, 37°C, variant G216K/K217G | Escherichia coli | |
0.53 | - |
IAA-Arg-Arg-p-nitroanilide | pH 6.5, 37°C, wild-type, purified from outer membrane | Escherichia coli | |
0.58 | - |
IAA-Arg-Arg-p-nitroanilide | pH 6.5, 37°C, wild-type, recombinant expressed | Escherichia coli | |
0.79 | - |
IAA-Arg-Arg-p-nitroanilide | pH 6.5, 37°C, variant G216K/K217G | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | integral membrane protease, outer membrane, membrane bound | Escherichia coli | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29000 | - |
SDS-PAGE | Escherichia coli |
33480 | - |
ESI-MS,positive-ion-mode electrospray ionization mass spectrometry | Escherichia coli |
33500 | - |
calculated from amino acid sequence | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Escherichia coli | cleaves peptides between two consecutive basic amino acids | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-aminobenzoyl-AKKA-3-[(2,4-dinitrophenyl)amino]-L-alanyl-Gly + H2O | - |
Escherichia coli | ? | - |
? | |
2-aminobenzoyl-ARKA-3-[(2,4-dinitrophenyl)amino]-L-alanyl-Gly + H2O | - |
Escherichia coli | ? | - |
? | |
2-aminobenzoyl-IRRA-3-[(2,4-dinitrophenyl)amino]-L-alanyl-Gly + H2O | - |
Escherichia coli | 2-aminobenzoyl-Ile-Arg + Arg-Ala-3-[(2,4-dinitrophenyl)amino]-L-alanyl-Gly | - |
? | |
aminobenzoyl-Ala-Lys-Lys-Ala-3-(dinitrophenyl)diaminopropionic acid-Gly + H2O | - |
Escherichia coli | ? | - |
? | |
IAA-Arg-Arg-p-nitroanilide + H2O | - |
Escherichia coli | ? | - |
? | |
additional information | cleaves peptides between two consecutive basic amino acids | Escherichia coli | ? | - |
? | |
o-aminobenzoyl-Ala-Arg-Arg-Ala-3-nitrotyrosine-NH2 + H2O | - |
Escherichia coli | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ompT | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
18 | - |
IAA-Arg-Arg-p-nitroanilide | pH 6.5, 37°C, variant G216K/K217G | Escherichia coli | |
24 | - |
IAA-Arg-Arg-p-nitroanilide | pH 6.5, 37°C, wild-type, purified from outer membrane and wild-type, recombinant expressed | Escherichia coli | |
96 | - |
o-aminobenzoyl-Ala-Arg-Arg-Ala-3-nitrotyrosine-NH2 | pH 6.5, 37°C, variant G216K/K217G | Escherichia coli | |
2280 | - |
o-aminobenzoyl-Ala-Arg-Arg-Ala-3-nitrotyrosine-NH2 | pH 6.5, 37°C, wild-type, purified from outer membrane | Escherichia coli | |
2460 | - |
o-aminobenzoyl-Ala-Arg-Arg-Ala-3-nitrotyrosine-NH2 | pH 6.5, 37°C, wild-type, recombinant expressed in Escherichia coli | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | - |
Escherichia coli |