Cloned (Comment) | Organism |
---|---|
procardosin B with and without plant-specific insert domain is expressed in Kluyveromyces lactis strain GG799 | Cynara cardunculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not inhibited by E-64, EDTA and Pefabloc | Cynara cardunculus | |
pepstatin A | - |
Cynara cardunculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0014 | - |
((7-methoxycoumarin-4-yl)acetyl)-Lys-Lys-Pro-Ala-Glu-Phe-Phe-Ala-Leu-Lys-(2,4-dinitrophenyl) | native mature enzyme, at pH 4.0 and 37°C | Cynara cardunculus | |
0.0087 | - |
((7-methoxycoumarin-4-yl)acetyl)-Lys-Lys-Pro-Ala-Glu-Phe-Phe-Ala-Leu-Lys-(2,4-dinitrophenyl) | recombinant proenzyme without plant-specific insert domain, at pH 4.0 and 37°C | Cynara cardunculus | |
0.0094 | - |
((7-methoxycoumarin-4-yl)acetyl)-Lys-Lys-Pro-Ala-Glu-Phe-Phe-Ala-Leu-Lys-(2,4-dinitrophenyl) | recombinant proenzyme with plant-specific insert domain, at pH 4.0 and 37°C | Cynara cardunculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cynara cardunculus | Q9XFX4 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | two putative glycosylation sites at N139 and N252 | Cynara cardunculus |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
((7-methoxycoumarin-4-yl)acetyl)-Lys-Lys-Pro-Ala-Glu-Phe-Phe-Ala-Leu-Lys-(2,4-dinitrophenyl) + H2O | - |
Cynara cardunculus | ? | - |
? | |
kappa-casein + H2O | - |
Cynara cardunculus | para-kappa-casein + ? | - |
? | |
additional information | the enzyme shows a preference for accommodating large hydrophobic amino acids at position P1, such as phenylalanine, tyrosine, and leucine. In P1', the enzyme prefers rather small amino acids like valine, alanine, and serine | Cynara cardunculus | ? | - |
? | |
skimmed milk + H2O | - |
Cynara cardunculus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 48000, recombinant procardosin B without plant-specific insert domain, SDS-PAGE | Cynara cardunculus |
Synonyms | Comment | Organism |
---|---|---|
cardosin B | - |
Cynara cardunculus |
PCB | proenzyme | Cynara cardunculus |
procardosin B | proenzyme | Cynara cardunculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Cynara cardunculus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 60 | the enzyme has more than 50% activity in a temperature range of 30 to 60°C | Cynara cardunculus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
19.2 | - |
((7-methoxycoumarin-4-yl)acetyl)-Lys-Lys-Pro-Ala-Glu-Phe-Phe-Ala-Leu-Lys-(2,4-dinitrophenyl) | recombinant proenzyme with plant-specific insert domain, at pH 4.0 and 37°C | Cynara cardunculus | |
24 | - |
((7-methoxycoumarin-4-yl)acetyl)-Lys-Lys-Pro-Ala-Glu-Phe-Phe-Ala-Leu-Lys-(2,4-dinitrophenyl) | native mature enzyme, at pH 4.0 and 37°C | Cynara cardunculus | |
36.5 | - |
((7-methoxycoumarin-4-yl)acetyl)-Lys-Lys-Pro-Ala-Glu-Phe-Phe-Ala-Leu-Lys-(2,4-dinitrophenyl) | recombinant proenzyme without plant-specific insert domain, at pH 4.0 and 37°C | Cynara cardunculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
- |
Cynara cardunculus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 5.5 | the enzyme has more than 50% activity in a pH range of 3.0-5.5 | Cynara cardunculus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2000 | - |
((7-methoxycoumarin-4-yl)acetyl)-Lys-Lys-Pro-Ala-Glu-Phe-Phe-Ala-Leu-Lys-(2,4-dinitrophenyl) | recombinant proenzyme with plant-specific insert domain, at pH 4.0 and 37°C | Cynara cardunculus | |
4200 | - |
((7-methoxycoumarin-4-yl)acetyl)-Lys-Lys-Pro-Ala-Glu-Phe-Phe-Ala-Leu-Lys-(2,4-dinitrophenyl) | recombinant proenzyme without plant-specific insert domain, at pH 4.0 and 37°C | Cynara cardunculus | |
17100 | - |
((7-methoxycoumarin-4-yl)acetyl)-Lys-Lys-Pro-Ala-Glu-Phe-Phe-Ala-Leu-Lys-(2,4-dinitrophenyl) | native mature enzyme, at pH 4.0 and 37°C | Cynara cardunculus |