Literature summary for 3.4.23.32 extracted from

Fujinaga, M.; Cherney, M.M.; Oyama, H.; Oda, K.; James, M.N.
The molecular structure and catalytic mechanism of a novel carboxyl peptidase from Scytalidium lignicolum (2004), Proc. Natl. Acad. Sci. USA, 101, 3364-3369.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
purified enzyme complexed with angiotensin II, hanging drop method, room temperature, from 42% saturated ammonium sulfate, 0.1 M sodium acetate, pH 4.0, 10% v/v ethylene glycol, crystals are soaked in cryosolution containing 30% glvcerol, 45% saturated ammonium sulfate,and 0.1 M sodium acetate, pH 4.0, heavy atom derivatizing, X-ray diffraction structure determination and analysis at 1.9-2.5 A resolution, molecular modeling and multiple isomorphous replacement phasing	Scytalidium lignicola

Crystallization (Comment)

Organism

purified enzyme complexed with angiotensin II, hanging drop method, room temperature, from 42% saturated ammonium sulfate, 0.1 M sodium acetate, pH 4.0, 10% v/v ethylene glycol, crystals are soaked in cryosolution containing 30% glvcerol, 45% saturated ammonium sulfate,and 0.1 M sodium acetate, pH 4.0, heavy atom derivatizing, X-ray diffraction structure determination and analysis at 1.9-2.5 A resolution, molecular modeling and multiple isomorphous replacement phasing

Scytalidium lignicola

Organism

Organism	UniProt	Comment	Textmining
Scytalidium lignicola	-	-	-

Reaction

Reaction	Comment	Organism	Reaction ID
Hydrolysis of proteins with broad specificity, cleaving Phe24-/-Phe, but not Leu15-Tyr and Phe25-Tyr in the B chain of insulin	active site structure and substrate binding structure and mechanism, hydrolysis mechanism	Scytalidium lignicola	a

Subunits

Subunits	Comment	Organism
More	molecular structure analysis	Scytalidium lignicola

Synonyms

Synonyms	Comment	Organism
More	the enzyme belongs to the eqolisin family of proteases	Scytalidium lignicola
SCP-B	-	Scytalidium lignicola
Scytalidium lignicolum pepstatin-insensitive carboxyl peptidase	-	Scytalidium lignicola

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Release 2023.1 (January 2023)