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Literature summary for 3.4.23.22 extracted from
- Protease of Endothia parasitica (1970), Methods Enzymol., 19, 436-445.
No PubMed abstract available
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Cryphonectria parasitica |
General Stability
| General Stability | Organism |
|---|---|
| Enzyme solution equilibrated against 0.01 M acetate buffer, pH 4.6, and frozen at -25°C to -30°C, may be lyophilized with no loss of activity | Cryphonectria parasitica |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | cysteine; HgCl2; iodoacetamide; NEM; not: sodium tetrathionate; PCMB; TLCK; TPCK | Cryphonectria parasitica |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34000 | 37500 | Endothia parasitica, gel filtration | Cryphonectria parasitica |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cryphonectria parasitica | - |
ATCC 14729 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Cryphonectria parasitica |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Cryphonectria parasitica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| casein + H2O | splits 29.0% of the peptide bonds | Cryphonectria parasitica | ? | - |
? |  |
| additional information | clots milk, not: benzyloxycarbonyl-L-Glu-L-Tyr | Cryphonectria parasitica | ? | - |
? | |
| Pepsin + H2O | splits 8.0% of the peptide bonds | Cryphonectria parasitica | ? | - |
? | |
| Rennin + H2O | splits 10.2% of the peptide bonds | Cryphonectria parasitica | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Cryphonectria parasitica |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3.8 | 4.5 | 50°C, maximal stability, 30% loss of activity after 30 min | Cryphonectria parasitica |
| 6.5 | - |
rapid loss of activity above | Cryphonectria parasitica |
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Release 2023.1 (January 2023)
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