BRENDA - Enzyme Database show
show all sequences of 3.4.22.B75

The deSUMOylase SENP7 promotes chromatin relaxation for homologous recombination DNA repair

Garvin, A.J.; Densham, R.M.; Blair-Reid, S.A.; Pratt, K.M.; Stone, H.R.; Weekes, D.; Lawrence, K.J.; Morris, J.R.; EMBO Rep. 14, 975-983 (2013)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
recombinant expression of N-terminally FLAG-tagged wild-type and mutant enzymes in HEK-293 cells
Homo sapiens
Engineering
Amino acid exchange
Commentary
Organism
C992A
site-directed mutagenesis, inactive catalytic mutant
Homo sapiens
additional information
enzyme knockout by siRNA in HeLa cells, complementation of knockdown with siRNA-resistant Flag-SENP7
Homo sapiens
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
nucleus
-
Homo sapiens
5634
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Homo sapiens
the enzyme SENP7 interacts with the chromatin repressive KRAB-associated protein 1 through heterochromatin protein 1 alpha, HP1alapha. Enzyme SENP7 contains a conserved HP1-box, PxVxL, required for interaction with HP1
?
-
-
-
SUMOylated KRAB-associated protein 1 + H2O
Homo sapiens
enzyme SENP7 promotes the removal of SUMO2/3 from KRAB-associated protein 1
KRAB-associated protein 1 + SUMO 2 + SUMO3
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
the enzyme SENP7 interacts with the chromatin repressive KRAB-associated protein 1 through heterochromatin protein 1 alpha, HP1alapha. Enzyme SENP7 contains a conserved HP1-box, PxVxL, required for interaction with HP1
731715
Homo sapiens
?
-
-
-
-
polySUMO2 KRAB-associated protein 1 + H2O
enzyme SENP7 promotes the removal of SUMO2 from KRAB-associated protein 1
731715
Homo sapiens
KRAB-associated protein 1 + SUMO 2 + SUMO3
-
-
-
?
SUMOylated KRAB-associated protein 1 + H2O
enzyme SENP7 promotes the removal of SUMO2/3 from KRAB-associated protein 1
731715
Homo sapiens
KRAB-associated protein 1 + SUMO 2 + SUMO3
-
-
-
?
Cloned(Commentary) (protein specific)
Commentary
Organism
recombinant expression of N-terminally FLAG-tagged wild-type and mutant enzymes in HEK-293 cells
Homo sapiens
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
C992A
site-directed mutagenesis, inactive catalytic mutant
Homo sapiens
additional information
enzyme knockout by siRNA in HeLa cells, complementation of knockdown with siRNA-resistant Flag-SENP7
Homo sapiens
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
nucleus
-
Homo sapiens
5634
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Homo sapiens
the enzyme SENP7 interacts with the chromatin repressive KRAB-associated protein 1 through heterochromatin protein 1 alpha, HP1alapha. Enzyme SENP7 contains a conserved HP1-box, PxVxL, required for interaction with HP1
?
-
-
-
SUMOylated KRAB-associated protein 1 + H2O
Homo sapiens
enzyme SENP7 promotes the removal of SUMO2/3 from KRAB-associated protein 1
KRAB-associated protein 1 + SUMO 2 + SUMO3
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
the enzyme SENP7 interacts with the chromatin repressive KRAB-associated protein 1 through heterochromatin protein 1 alpha, HP1alapha. Enzyme SENP7 contains a conserved HP1-box, PxVxL, required for interaction with HP1
731715
Homo sapiens
?
-
-
-
-
polySUMO2 KRAB-associated protein 1 + H2O
enzyme SENP7 promotes the removal of SUMO2 from KRAB-associated protein 1
731715
Homo sapiens
KRAB-associated protein 1 + SUMO 2 + SUMO3
-
-
-
?
SUMOylated KRAB-associated protein 1 + H2O
enzyme SENP7 promotes the removal of SUMO2/3 from KRAB-associated protein 1
731715
Homo sapiens
KRAB-associated protein 1 + SUMO 2 + SUMO3
-
-
-
?
General Information
General Information
Commentary
Organism
malfunction
homologous recombination is affected in SENP7 knockout cells
Homo sapiens
metabolism
DNA damage-associated remodelling requires SENP7, overview
Homo sapiens
physiological function
enzyme SENP7 promotes the removal of SUMO2/3 from KRAB-associated protein 1 and regulates the interaction of the chromatin remodeler CHD3 with chromatin. In the presence of CHD3, SENP7 is required for chromatin relaxation in response to DNA damage, for homologous recombination repair and for cellular resistance to DNA-damaging agents. DeSUMOylation by SENP7 is required to promote a permissive chromatin environment for DNA repair. Enzyme SENP7 is required for localized euchromatin relaxation
Homo sapiens
General Information (protein specific)
General Information
Commentary
Organism
malfunction
homologous recombination is affected in SENP7 knockout cells
Homo sapiens
metabolism
DNA damage-associated remodelling requires SENP7, overview
Homo sapiens
physiological function
enzyme SENP7 promotes the removal of SUMO2/3 from KRAB-associated protein 1 and regulates the interaction of the chromatin remodeler CHD3 with chromatin. In the presence of CHD3, SENP7 is required for chromatin relaxation in response to DNA damage, for homologous recombination repair and for cellular resistance to DNA-damaging agents. DeSUMOylation by SENP7 is required to promote a permissive chromatin environment for DNA repair. Enzyme SENP7 is required for localized euchromatin relaxation
Homo sapiens
Other publictions for EC 3.4.22.B75
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
731599
Gonzalez-Prieto
c-Myc is targeted to the prote ...
Homo sapiens
Cell Cycle
14
1859-1872
2015
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4
4
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731614
Romeo
The SENP7 SUMO-protease presen ...
Mus musculus
Cell Rep.
10
771-782
2015
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1
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3
3
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732884
Alegre
Structural insights into the S ...
Homo sapiens
Protein Sci.
23
433-441
2014
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731715
Garvin
The deSUMOylase SENP7 promotes ...
Homo sapiens
EMBO Rep.
14
975-983
2013
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1
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2
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3
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732566
Maison
The SUMO protease SENP7 is a c ...
Mus musculus
Nat. Struct. Mol. Biol.
19
458-460
2012
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2
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732812
Bawa-Khalfe
Differential expression of SUM ...
Homo sapiens
Proc. Natl. Acad. Sci. USA
109
17466-17471
2012
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2
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714083
Shen
Characterization of SENP7, a S ...
Homo sapiens
Biochem. J.
421
223-230
2009
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1
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714081
Drag
Activity profiling of human de ...
Homo sapiens
Biochem. J.
409
461-469
2008
2
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715494
Lima
Structure of the human SENP7 c ...
Homo sapiens
J. Biol. Chem.
283
32045-32055
2008
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