Literature summary for 3.4.22.B6 extracted from

Ferrara, T.F.; Schneider, V.K.; Kishi, L.T.; Carmona, A.K.; Alves, M.F.; Belasque-Junior, J.; Rosa, J.C.; Hunter, W.B.; Henrique-Silva, F.; Soares-Costa, A.
Characterization of a recombinant cathepsin B-like cysteine peptidase from Diaphorina citri Kuwayama (Hemiptera Liviidae) A putative target for control of Citrus Huanglongbing (2015), PLoS ONE, 10, e0145132 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Pichia pastoris	Diaphorina citri

Inhibitors

Inhibitors	Comment	Organism	Structure
CA-074	-	Diaphorina citri
CaneCPI-4	-	Diaphorina citri
E64	-	Diaphorina citri

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00613	-	Z-Arg-Arg-7-amido-4-methylcoumarin	pH 8.0, 37°C	Diaphorina citri
0.0235	-	Z-Phe-Arg-7-amido-4-methylcoumarin	pH 8.0, 37°C	Diaphorina citri

Organism

Organism	UniProt	Comment	Textmining
Diaphorina citri	A0A0U4DX43	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant prrotein	Diaphorina citri

Source Tissue

Source Tissue	Comment	Organism	Textmining
adult	the expression of DCcathB in nymph and adult is approximately 9fold greater than in egg	Diaphorina citri	-
gut	the expression in the gut is 175fold and 3333fold higher than in the remaining tissues and in the head, respectively	Diaphorina citri	-
nymph	the expression of DCcathB in nymph and adult is approximately 9fold greater than in egg	Diaphorina citri	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Z-Arg-Arg-7-amido-4-methylcoumarin + H2O	-	Diaphorina citri	Z-Arg-Arg + 7-amino-4-methylcoumarin	-	?
Z-Phe-Arg-7-amido-4-methylcoumarin + H2O	-	Diaphorina citri	Z-Phe-Arg + 7-amino-4-methylcoumarin	-	?

Subunits

Subunits	Comment	Organism
?	x * 50000, SDS-PAGE, x * 41900, calculated from sequence	Diaphorina citri

Synonyms

Synonyms	Comment	Organism
LOC103512321	-	Diaphorina citri

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.00000005	-	pH 8.0, 37°C	Diaphorina citri	CaneCPI-4
0.000000095	-	pH 8.0, 37°C	Diaphorina citri	CA-074
0.000014	-	pH 8.0, 37°C	Diaphorina citri	E64

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Release 2023.1 (January 2023)