BRENDA - Enzyme Database show
show all sequences of 3.4.22.B6

Nicotinamide inhibits the lysosomal cathepsin B-like protease and kills African trypanosomes

Unciti-Broceta, J.D.; Maceira, J.; Morales, S.; Garcia-Perez, A.; Munoz-Torres, M.E.; Garcia-Salcedo, J.A.; J. Biol. Chem. 288, 10548-10557 (2013)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
recombinant expression in Pichia pastoris
Trypanosoma brucei
Inhibitors
Inhibitors
Commentary
Organism
Structure
nicotinamide
complete inhibition at 25 mM
Trypanosoma brucei
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
lysosome
-
Trypanosoma brucei
5764
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
42000
-
x * 42000, recombinant zymogen, SDS-PAGE
Trypanosoma brucei
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Trypanosoma brucei
Q6R7Z5
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Z-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
732089
Trypanosoma brucei
Z-Arg-Arg + 7-amino-4-methylcoumarin
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 42000, recombinant zymogen, SDS-PAGE
Trypanosoma brucei
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Trypanosoma brucei
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
assay at
Trypanosoma brucei
Cloned(Commentary) (protein specific)
Commentary
Organism
recombinant expression in Pichia pastoris
Trypanosoma brucei
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
nicotinamide
complete inhibition at 25 mM
Trypanosoma brucei
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
lysosome
-
Trypanosoma brucei
5764
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
42000
-
x * 42000, recombinant zymogen, SDS-PAGE
Trypanosoma brucei
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Z-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
732089
Trypanosoma brucei
Z-Arg-Arg + 7-amino-4-methylcoumarin
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 42000, recombinant zymogen, SDS-PAGE
Trypanosoma brucei
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Trypanosoma brucei
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
assay at
Trypanosoma brucei
General Information
General Information
Commentary
Organism
malfunction
inhibition of the lysosomal cathepsin B-like protease blocks endocytosis causing cell death. Nicotinamide causes cytokinesis defects and cell cycle arrest at G2 phase
Trypanosoma brucei
physiological function
cathepsin B-like is a lysosomal protease responsible for degradation of host-derived growth factors, such as transferrin, in the bloodstream form of Trypanosoma brucei
Trypanosoma brucei
General Information (protein specific)
General Information
Commentary
Organism
malfunction
inhibition of the lysosomal cathepsin B-like protease blocks endocytosis causing cell death. Nicotinamide causes cytokinesis defects and cell cycle arrest at G2 phase
Trypanosoma brucei
physiological function
cathepsin B-like is a lysosomal protease responsible for degradation of host-derived growth factors, such as transferrin, in the bloodstream form of Trypanosoma brucei
Trypanosoma brucei
Other publictions for EC 3.4.22.B6
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
732089
Unciti-Broceta
Nicotinamide inhibits the lyso ...
Trypanosoma brucei
J. Biol. Chem.
288
10548-10557
2013
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2
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731769
Ni
Cathepsin B-like and hemoglobi ...
Angiostrongylus cantonensis
Exp. Parasitol.
131
433-441
2012
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732606
Cheng
Cloning and characterization o ...
Angiostrongylus cantonensis
Parasitol. Res.
110
2413-2422
2012
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2
1
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1
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2
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3
3
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732686
Rieux
Eimeripain, a cathepsin B-like ...
Eimeria tenella
PLoS ONE
7
e31914
2012
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2
2
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718224
Thul
-
Cathepsin B-like protease from ...
Capsicum frutescens, Nicotiana rustica
Plant OMICS
4
120-125
2011
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708228
Chi
N-glycosylation at non-canonic ...
Callosobruchus maculatus
Comp. Biochem. Physiol. B
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40-47
2010
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Kerr
Crystal Structures of TbCatB a ...
Trypanosoma brucei
PLoS Negl. Trop. Dis.
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e701
2010
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707833
Miranda-Miranda
-
Molecular cloning and expressi ...
Caenorhabditis elegans
Biotechnology
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2009
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708792
Doleckova
The functional expression and ...
Trichobilharzia regenti
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201-211
2009
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Kim
Immunodominant antigens in Nae ...
Naegleria fowleri
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710672
De Vries
An AC-5 cathepsin B-like prote ...
Haemonchus contortus
Vet. Res.
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41
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679598
Mendoza-Palomares
Molecular and biochemical char ...
Trypanosoma congolense
Eukaryot. Cell
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684-697
2008
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4
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4
1
1
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680163
Koo
Functional expression of an in ...
Callosobruchus maculatus
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2008
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Ahn
Seven-up facilitates insect co ...
Callosobruchus maculatus
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663785
Zhao
Expression of the Helicoverpa ...
Helicoverpa armigera
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39-46
2005
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664706
Mottram
Cysteine peptidases as virulen ...
Leishmania amazonensis, Leishmania major, Leishmania mexicana
Curr. Opin. Microbiol.
7
375-381
2004
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666285
Jasmer
mRNA sequences for Haemonchus ...
Haemonchus contortus
Mol. Biochem. Parasitol.
137
297-305
2004
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666453
Graber
Cathepsin B-like proteolysis a ...
Homo sapiens
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2004
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647655
Chan
Expression and alteration of t ...
Leishmania major
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1999
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Sakanari
Leishmania major: comparison o ...
Leishmania major
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1997
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647656
Selzer
Leishmania major: molecular mo ...
Leishmania major
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87
212-221
1997
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4
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4
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