Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Drosophila melanogaster |
Protein Variants | Comment | Organism |
---|---|---|
C314A | inactive | Drosophila melanogaster |
S845E | the mutation causes a small but reproducible increase in the activation of calpain B | Drosophila melanogaster |
T747E | the mutation causes a large increase in the activation of calpain B | Drosophila melanogaster |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | a 6.4 mM free Ca2+ concentration is required for the half maximal activation of calpain B | Drosophila melanogaster |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila melanogaster | Q9VT65 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | phosphorylation elevates the Ca2+ sensitivity of the protease. The activation of the extracellular signal-regulated protein kinase pathway by extracellular signals results in the phosphorylation and activation of calpain B in fruit flies | Drosophila melanogaster |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Drosophila melanogaster |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
SCHNEIDER-2 cell | - |
Drosophila melanogaster | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
microtubule-associated protein 2c + H2O | - |
Drosophila melanogaster | ? | - |
? | |
N-succinyl-L-Leu-L-Tyr-7-amido-4-methylcoumarin + H2O | - |
Drosophila melanogaster | N-succinyl-L-Leu-L-Tyr + 7-amino-4-methyl-coumarin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
calpain B | - |
Drosophila melanogaster |