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Literature summary for 3.4.22.B37 extracted from
- Characterization of two recombinant Drosophila calpains. CALPA and a novel homolog, CALPB (1999), J. Biol. Chem., 274, 23893-23900.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no activation by phosphatidylinositol 4,5-diphosphate, phosphatidylinositol 4-monophosphate, phosphatidylinositol or phosphatidic acid. N-terminal autolysis in a Ca2+-dependent manner which runs parallel with the enzyme activation | Drosophila melanogaster |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Drosophila melanogaster |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | in Schneider S2 cells, with increasing Ca2+ concentrations the enzyme adheres to intracellular membranes | Drosophila melanogaster | 5737 | - |
| membrane | with increasing Ca2+ concentrations the enzyme adheres to intracellular membranes | Drosophila melanogaster | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | half-maximal activation at 3.28 mM for the soluble calpain B and 4.38 mM for the renatured calpain B | Drosophila melanogaster |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 90134 | - |
x * 90134, calculation from nucleotide sequence | Drosophila melanogaster |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Drosophila melanogaster | - |
- |
- |
| Drosophila melanogaster | Q9VT65 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Drosophila melanogaster |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | N-terminal autolysis in a Ca2+-dependent manner which runs parallel with the enzyme activation | Drosophila melanogaster | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 90134, calculation from nucleotide sequence | Drosophila melanogaster |
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