BRENDA - Enzyme Database
show all sequences of 3.4.22.B36

Characterization of two recombinant Drosophila calpains. CALPA and a novel homolog, CALPB

Jekely, G.; Friedrich, P.; J. Biol. Chem. 274, 23893-23900 (1999)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
phosphatidic acid
activates
Drosophila melanogaster
phosphatidylinositol
activates
Drosophila melanogaster
phosphatidylinositol 4,5-bisphosphate
activates
Drosophila melanogaster
phosphatidylinositol 4-monophosphate
activates
Drosophila melanogaster
Cloned(Commentary)
Commentary
Organism
expression of wild-type and truncated enzyme form, lacking the CALPA-specific unique insertion region
Drosophila melanogaster
Engineering
Amino acid exchange
Commentary
Organism
additional information
the truncated enzyme form lacking the CALPA-specific unique insertion region. Although it lacks the 16-amino acid long putative membrane-anchoring segment, its activation by phospholipids is similar to that of the full-length CALPA protein
Drosophila melanogaster
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
activates, half-maximal activation of the full-length enzyme at 2.18 mM and for the truncated enzyme at 3.23 mM
Drosophila melanogaster
Mn2+
slight activation
Drosophila melanogaster
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Drosophila melanogaster
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
casein + H2O
-
648418
Drosophila melanogaster
hydrolyzed casein
-
-
-
?
additional information
the enzyme undergoes N-terminal autolysis in a Ca2+-dependent manner
648418
Drosophila melanogaster
?
-
-
-
-
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
phosphatidic acid
activates
Drosophila melanogaster
phosphatidylinositol
activates
Drosophila melanogaster
phosphatidylinositol 4,5-bisphosphate
activates
Drosophila melanogaster
phosphatidylinositol 4-monophosphate
activates
Drosophila melanogaster
Cloned(Commentary) (protein specific)
Commentary
Organism
expression of wild-type and truncated enzyme form, lacking the CALPA-specific unique insertion region
Drosophila melanogaster
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
the truncated enzyme form lacking the CALPA-specific unique insertion region. Although it lacks the 16-amino acid long putative membrane-anchoring segment, its activation by phospholipids is similar to that of the full-length CALPA protein
Drosophila melanogaster
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
activates, half-maximal activation of the full-length enzyme at 2.18 mM and for the truncated enzyme at 3.23 mM
Drosophila melanogaster
Mn2+
slight activation
Drosophila melanogaster
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
casein + H2O
-
648418
Drosophila melanogaster
hydrolyzed casein
-
-
-
?
additional information
the enzyme undergoes N-terminal autolysis in a Ca2+-dependent manner
648418
Drosophila melanogaster
?
-
-
-
-
Other publictions for EC 3.4.22.B36
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
732480
Fontenele
Calpain A modulates Toll respo ...
Drosophila melanogaster
Mol. Biol. Cell
24
2966-2980
2013
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1
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1
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1
1
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1
1
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1
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3
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2
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2
2
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709802
Fontenele
The Ca2+-dependent protease ca ...
Drosophila melanogaster
Mech. Dev.
126
737-751
2009
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2
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648417
Stapleton
-
A Drosophila full-length cDNA ...
Drosophila melanogaster, Drosophila melanogaster Berkeley
Genome Biol.
3
research0080.1-0080.8
2002
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490160
Adams
The genome sequence of Drosoph ...
Drosophila melanogaster, Drosophila melanogaster Berkeley
Science
287
2185-2195
2000
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3
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648418
Jekely
Characterization of two recomb ...
Drosophila melanogaster
J. Biol. Chem.
274
23893-23900
1999
4
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1
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1
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2
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6
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648414
Theopold
CalpA, a Drosophila calpain ho ...
Drosophila melanogaster, Drosophila melanogaster Canton-S
Mol. Cell. Biol.
15
824-834
1995
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6
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9
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648415
Emori
Calpain localization changes i ...
Drosophila melanogaster, Drosophila melanogaster Canton-S
J. Biol. Chem.
269
25137-25142
1994
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