Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Gu-HCl | 500 mM reduces cleavage to 60%, 1 M reduces cleavage to 0% | Saccharomyces cerevisiae | |
additional information | no inhibitory effects are observed with Triton X100 (1 M), imidazole (300 mM), reduced glutathione (20 mM), maltose (20 mM), glycerol (20% v/v), ethylene glycol (20% v/v), sucrose (20% w/v), ethanol (10% v/v) | Saccharomyces cerevisiae | |
NaCl | 500 mM reduces cleavage to 60%, 1 M reduces cleavage to 30% | Saccharomyces cerevisiae | |
Urea | 2 M reduces cleavage to 95%, 3 M reduces cleavage to 5% | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31000 | - |
x * 31000, SDS-PAGE | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | Q02724 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Storage Stability | Organism |
---|---|
-70°C, 50% glycerol, 75 mM Tris-HCl pH 8.0, 0.5 mM DTT, 1 mM EDTA, 6 months, no detectable decay of activity | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
small ubiquitin-like modifier protein + H2O | - |
Saccharomyces cerevisiae | ? | Ulp1 catalyzes the proteolytic processing of SUMO to its mature form | ? | |
SUMO-GFP fusion substrate + H2O | pH 8.0, 25°C, in the presence of 5 mM 2-mercaptoethanol | Saccharomyces cerevisiae | SUMO + GFP | - |
? | |
SUMO-MMP13 + H2O | cleavage occurs only to 60% | Saccharomyces cerevisiae | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 31000, SDS-PAGE | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
SUMO protease 1 | - |
Saccharomyces cerevisiae |
Ulp1 | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | 37 | - |
Saccharomyces cerevisiae |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | 37 | - |
Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 9 | - |
Saccharomyces cerevisiae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | - |
Saccharomyces cerevisiae |