Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
inactive Bid + H2O | Mus musculus | cleavage at amino acid 59 | active tBid + ? | - |
? | |
pro-caspase-3 + H2O | Mus musculus | - |
active caspase-3 + caspase-3 propeptide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
embryonic fibroblast | - |
Mus musculus | - |
FL5.12 cell | - |
Mus musculus | - |
MEF cell | - |
Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
inactive Bid + H2O | cleavage at amino acid 59 | Mus musculus | active tBid + ? | - |
? | |
inactive Bid + H2O | cleavage at amino acid 59. Substrate mutants BidD98A and BidD75A are cleaved by caspase-9, mutant BidD59A is not cleaved | Mus musculus | active tBid + ? | - |
? | |
pro-caspase-3 + H2O | - |
Mus musculus | active caspase-3 + caspase-3 propeptide | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mus musculus |
General Information | Comment | Organism |
---|---|---|
malfunction | introduction of a dominant negative Casp9 inhibits mitochondrial remodeling without affecting the release of cytochrome c | Mus musculus |
metabolism | caspase-9, caspase-3 and caspase-7 have distinct roles during intrinsic apoptosis, which is is a form of programmed cell death that is regulated by the Bcl-2 family and caspase family of proteins. The caspase cascade is responsible for executing cell death following cytochrome c release. Caspase-9 is required for mitochondrial morphological changes and reactive oxygen species production by cleaving and activating Bid into tBid. After activation by caspase-9, caspase-3 inhibits reactive oxygen species production and is required for efficient execution of apoptosis, while effector caspase-7 is required for apoptotic cell detachment | Mus musculus |
physiological function | caspase-9 is required for mitochondrial morphological changes and reactive oxygen species production by cleaving and activating Bid into tBid, it activates caspase-3 and is necessary to remodel the mitochondria during intrinsic apoptosis | Mus musculus |