Inhibitors | Comment | Organism | Structure |
---|---|---|---|
antipain | 0.01 mM 56% inhibition | Pelophylax ridibundus | |
Cd2+ | 5 mM, completely blocks activation of the enzyme by Ca2+ | Pelophylax ridibundus | |
Co2+ | 5 mM, completely blocks activation of the enzyme by Ca2+ | Pelophylax ridibundus | |
Cu2+ | 5 mM, completely blocks activation of the enzyme by Ca2+ | Pelophylax ridibundus | |
E-64 | 0.01 mM 89% inhibition | Pelophylax ridibundus | |
iodoacetamide | 1 mM, 64% inhibition | Pelophylax ridibundus | |
iodoacetic acid | 1 mM, 76% inhibition | Pelophylax ridibundus | |
leupeptin | 0.01 mM 85% inhibition | Pelophylax ridibundus | |
NEM | 1 mM, 93% inhibition | Pelophylax ridibundus | |
Ni2+ | 5 mM, completely blocks activation of the enzyme by Ca2+ | Pelophylax ridibundus | |
Zn2+ | 5 mM, completely blocks activation of the enzyme by Ca2+ | Pelophylax ridibundus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | 1 mM, decreases the Ca2+-requirement for maximal activity from 0.4 mM to 0.3 mM. Synergistic activating effect with Ca2+ | Pelophylax ridibundus | |
Ca2+ | half-maximal activity at 0.4 mM, maximal activity at 1.5 mM | Pelophylax ridibundus | |
Mg2+ | 1 mM decreases the Ca2+-requirement for maximal activity from 0.4 mM to 0.3 mM. Synergistic activating effect with Ca2+ | Pelophylax ridibundus | |
Mn2+ | 1 mM, decreases the half-maximal Ca2+-requirement from 0.4 mM to 0.1 mM, decreases the Ca2+-requirement for maximal activity from 1.5 mM to 1 mM | Pelophylax ridibundus | |
Sr2+ | 1 mM, decreases the half-maximal Ca2+-requirement from 0.4 mM to 0.1 mM, decreases the Ca2+-requirement for maximal activity from 1.5 mM to 1 mM. Synergistic activating effect with Ca2+ | Pelophylax ridibundus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
1 * 78000 + 1 * 28000, SDS-PAGE | Pelophylax ridibundus |
78000 | - |
1 * 78000 + 1 * 28000, SDS-PAGE | Pelophylax ridibundus |
105000 | - |
gel filtration | Pelophylax ridibundus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pelophylax ridibundus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pelophylax ridibundus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
skeletal muscle | - |
Pelophylax ridibundus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Pelophylax ridibundus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
casein + H2O | - |
Pelophylax ridibundus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 78000 + 1 * 28000, SDS-PAGE | Pelophylax ridibundus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Pelophylax ridibundus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
10 min, in absence of Ca2+, about 10% loss of activity | Pelophylax ridibundus |
60 | - |
10 min, in absence of Ca2+, about 90% loss of activity | Pelophylax ridibundus |