Cloned (Comment) | Organism |
---|---|
expression of His6-tagged or MBP-fused wild-type enzyme and of MBP-fused mutant HC-ProFINK protein in Escherichia coli strain BL21 (DE3) | zucchini yellow mosaic virus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | a point mutation in the highly conserved FRNK box produces the HC-ProFINK protein that shows no sRNA binding | zucchini yellow mosaic virus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
zucchini yellow mosaic virus | A1DU45 | polyprotein, sequence ID of mutant HC-ProFINK protein is A1DU46; ZYMV | - |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His6-tagged or MBP-fused wild-type enzyme and MBP-fused mutant HC-ProFINK protein from Escherichia coli strain BL21 (DE3) by affinity chromatography | zucchini yellow mosaic virus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | analysis of RNA-binding activity of HC-Pro by EMSA, overview | zucchini yellow mosaic virus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
HC-Pro | - |
zucchini yellow mosaic virus |
helper component-proteinase | - |
zucchini yellow mosaic virus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
sRNA binding assay at | zucchini yellow mosaic virus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
sRNA binding assay at | zucchini yellow mosaic virus |
General Information | Comment | Organism |
---|---|---|
malfunction | a point mutation in the highly conserved FRNK box produces the HC-ProFINK protein that shows no sRNA binding | zucchini yellow mosaic virus |
physiological function | HC-Pro is a helper component-proteinase which acts as a multifunctional protein in the potyviral life cycle. Apart from its proteolytic activity, HC-Pro has the capacity to bind duplex small RNAs | zucchini yellow mosaic virus |