Crystallization (Comment) | Organism |
---|---|
purified enzyme gingipain R2 in complex with fast acting, irreversible inhibitor H-D-Phe-Phe-Arg-chloromethylketone, activated by incubation for 30 min in 0.1 M HEPES, pH 8.0, 10 mM L-cysteine, 2.5 mM CaCl2, at 37°C, alkylation of the active site cysteine by addition of inhibitor, crystallization by sitting-drop vapour diffusion method at 20°C, different protein-inhibitor complex solution systems using PEG 8000 as precipitant for 10 mg/ml protein, X-ray diffraction structure determination at 2.9 A resolution and analysis | Porphyromonas gingivalis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-Phe-Phe-Arg-chloromethylketone | fast acting, irreversible alkylating inhibitor | Porphyromonas gingivalis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
protein + H2O | Porphyromonas gingivalis | - |
peptides | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Porphyromonas gingivalis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Porphyromonas gingivalis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
hydrolysis of proteins and small molecule substrates, with a preference for Arg in P1 | active site structure | Porphyromonas gingivalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
protein + H2O | - |
Porphyromonas gingivalis | peptides | - |
? |
Synonyms | Comment | Organism |
---|---|---|
gingipain R2 | - |
Porphyromonas gingivalis |
RGP-2 | - |
Porphyromonas gingivalis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Porphyromonas gingivalis | gingipain R2 isozyme II, isoelectric focusing | - |
5.1 |