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Literature summary for 3.4.22.35 extracted from

  • Ghosh, A.; Raha, S.
    Molecular and functional characterisation of a stress responsive cysteine protease, EhCP6 from Entamoeba histolytica (2015), Protein Expr. Purif., 109, 55-61.
    View publication on PubMed
Search for more literature for 3.4.22.35

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Entamoeba histolytica

Inhibitors

Inhibitors Comment Organism Structure
E-64
-
 Entamoeba histolytica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
 x * 25000, mature enzyme, SDS-PAGE Entamoeba histolytica

Organism

Organism UniProt Comment Textmining
Entamoeba histolytica C4LSI6
-
-
Entamoeba histolytica HM1:IMSS C4LSI6
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-NTA resin column chromatography, and gel filtration Entamoeba histolytica

Renatured (Commentary)

Renatured (Comment) Organism
using 50 mM Tris pH 6.8,150 mM NaCl, 5% (v/v) glycerol, 0.5 mM oxidised glutathione, 3 mM reduced glutathione, at 4°C Entamoeba histolytica

Source Tissue

Source Tissue Comment Organism Textmining
trophozoite
-
 Entamoeba histolytica
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
azocasein + H2O
-
 Entamoeba histolytica ?
-
 ?
azocasein + H2O
-
 Entamoeba histolytica HM1:IMSS ?
-
 ?
Gelatin + H2O
-
 Entamoeba histolytica ?
-
 ?
Gelatin + H2O
-
 Entamoeba histolytica HM1:IMSS ?
-
 ?

Subunits

Subunits Comment Organism
? x * 25000, mature enzyme, SDS-PAGE Entamoeba histolytica

Synonyms

Synonyms Comment Organism
CP6
-
 Entamoeba histolytica
cysteine protease 6
-
 Entamoeba histolytica

Expression

Organism Comment Expression
Entamoeba histolytica cysteine protease 6 is upregulated in response to heat shock and during pathogen invasion of the host tissue up