BRENDA - Enzyme Database
show all sequences of 3.4.22.25

Structural characterization of the papaya cysteine proteinases at low pH

Huet, J.; Looze, Y.; Bartik, K.; Raussens, V.; Wintjens, R.; Boussard, P.; Biochem. Biophys. Res. Commun. 341, 620-626 (2006)

Data extracted from this reference:

Application
Application
Commentary
Organism
nutrition
the enzyme needs to be protected from acid denaturation and proteolysis in the gut after oral administration to be effective as cysteine protease
Carica papaya
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
extracellular
latex
Carica papaya
-
-
Organism
Organism
UniProt
Commentary
Textmining
Carica papaya
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
native enzyme from latex by ion exchange and adsorption chromatography, and gel filtration
Carica papaya
Source Tissue
Source Tissue
Commentary
Organism
Textmining
latex
-
Carica papaya
-
Synonyms
Synonyms
Commentary
Organism
glycine endopeptidase
-
Carica papaya
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
additional information
-
the enzyme undergoes a conformational transition at low pH and 37°C, that instantaneously and irreversibly converts the native form into molten globules, which are unstable and rapidly degraded by pepsin
Carica papaya
Application (protein specific)
Application
Commentary
Organism
nutrition
the enzyme needs to be protected from acid denaturation and proteolysis in the gut after oral administration to be effective as cysteine protease
Carica papaya
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
extracellular
latex
Carica papaya
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
native enzyme from latex by ion exchange and adsorption chromatography, and gel filtration
Carica papaya
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
latex
-
Carica papaya
-
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
additional information
-
the enzyme undergoes a conformational transition at low pH and 37°C, that instantaneously and irreversibly converts the native form into molten globules, which are unstable and rapidly degraded by pepsin
Carica papaya
Other publictions for EC
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
753097
Liu
Papain-like cysteine protease ...
Carica papaya
BMC Genomics
19
26
2018
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733902
Karnjanapratum
Glycyl endopeptidase from papa ...
Carica papaya
Food Chem.
165
403-411
2014
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679571
Chaiwut
-
Solid-to-solid peptide synthes ...
Carica papaya
Enzyme Microb. Technol.
40
954-960
2007
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667431
Huet
Structural characterization of ...
Carica papaya
Biochem. Biophys. Res. Commun.
341
620-626
2006
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668767
Buttle
-
Glycyl endopeptidase ...
Carica papaya
Handbook of Proteolytic Enzymes (Barrett, A.J., Rawlings, N.D., Woessner, J.F., eds)
2
1132-1134
2004
1
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652619
Azarkan
Fractionation and purification ...
Carica papaya
J. Chromatogr. B
790
229-238
2003
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651248
Visal
The proregion of papaya protei ...
Carica papaya
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434
401-405
1998
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30148
Dekeyser
Kinetic constants for the hydr ...
Carica papaya
Arch. Biochem. Biophys.
320
375-379
1995
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30150
O'Hara
Crystal structure of glycyl en ...
Carica papaya
Biochemistry
34
13190-13195
1995
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30151
Thomas
The structural origins of the ...
Carica papaya
Biochem. J.
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1995
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30155
Taylor
Expression of the pro-regions ...
Carica papaya
Biochem. Soc. Trans.
23
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1995
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30146
Buttle
Glycyl endopeptidase ...
Carica papaya
Methods Enzymol.
244
539-555
1994
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30153
Thomas
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Carica papaya
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30147
Sumner
Factors effecting the thermost ...
Carica papaya
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30144
Buttle
Selective cleavage of glycyl b ...
Carica papaya
FEBS Lett.
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1990
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30145
Buttle
Interactions of papaya protein ...
Carica papaya
FEBS Lett.
262
58-60
1990
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30152
Topham
Three-dimensional structure an ...
Carica papaya
Biochem. Soc. Trans.
18
934-935
1990
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30143
Ritonja
Papaya proteinase IV amino aci ...
Carica papaya
FEBS Lett.
258
109-112
1989
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30149
Buttle
Affinity purification of the n ...
Carica papaya
Biochem. J.
261
469-476
1989
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30154
Goodenough
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Chromatographic and electropho ...
Carica papaya
Phytochemistry
26
75-79
1987
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30142
Polgar
Isolation of highly active pap ...
Carica papaya
Biochim. Biophys. Acta
658
262-269
1981
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