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Literature summary for 3.4.22.15 extracted from

  • Tamhane, T.; Lllukkumbura, R.; Lu, S.; Maelandsmo, G.M.; Haugen, M.H.; Brix, K.
    Nuclear cathepsin L activity is required for cell cycle progression of colorectal carcinoma cells (2016), Biochimie, 122, 208-218 .
    View publication on PubMed
Search for more literature for 3.4.22.15

Inhibitors

Inhibitors Comment Organism Structure
E-64 a broad-spectrum cysteine peptidase inhibitor Homo sapiens
additional information endogenous cysteine peptidase inhibitors like stefin B act in safe-guarding mammalian cells by their presence in the cyto- or nucleoplasm, and thus protect from activities of proteolytic enzymes leaking into the cytoplasm upon, for instance, non-intended rupture of endolysosomes in pathological situations Homo sapiens
N-benzyloxycarbonyl-phenylalanyl-phenylalanine-fluoromethyl ketone an irreversible cathepsin inhibitor Homo sapiens
Stefin B a potent cathepsin L inhibitor, colorectal carcinoma cells reveal only very faint amounts of immunolabeled stefin B within their nuclei, while it is prominently present within the cytoplasm of Caco-2 and SW-620 cells, and mostly associated with the cytoplasmic face of vesicles in HCT-116 cells Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
lysosome
-
 Homo sapiens 5764
-
nucleus
-
 Homo sapiens 5634
-
plasma membrane apical Homo sapiens 5886
-
vesicle in epithelial cells of normal colon tissue, cathepsin L-specific fluorescence signals are detected within vesicles that are abundant at the apical pole, whereas vesicles containing cathepsin L are scattered throughout the cytoplasm in adenocarcinoma cells Homo sapiens 31982
-

Organism

Organism UniProt Comment Textmining
Homo sapiens P07711
-
-

Source Tissue

Source Tissue Comment Organism Textmining
adenocarcinoma cell
-
 Homo sapiens
-
Caco-2 cell
-
 Homo sapiens
-
colon
-
 Homo sapiens
-
colorectal cancer cell
-
 Homo sapiens
-
epithelial cell
-
 Homo sapiens
-
HCT-116 cell tial expression of cathepsin L during the cell cycle of HCT116 cells.Cathepsin L amounts are highest in nuclear fractions compared to lysosomal preparations of G1/G0-arrested cells, whereas lysosomal levels of cathepsin L protein exceed those of the nuclear fractions when cells are inspected in either S or G2/M phase Homo sapiens
-
IEC-6 cell
-
 Homo sapiens
-
intestine
-
 Homo sapiens
-
additional information enzyme expression levels in normal epithelial and in proliferating cancer cells, overview Homo sapiens
-
SW-620 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens N-benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
-
 ?

Synonyms

Synonyms Comment Organism
cathepsin L1
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
 assay at Homo sapiens

General Information

General Information Comment Organism
physiological function nuclear cathepsin L activity is required for cell cycle progression of colorectal carcinoma cells. The activity of cathepsin L is high in the nucleus of colorectal carcinoma cells because of lacking stefin B inhibitory activity. Nuclear cathepsin L accelerates cell cycle progression of HCT116 cells. Cysteine cathepsins may play significant roles in carcinogenesis due to deregulated trafficking Homo sapiens