Cloned (Comment) | Organism |
---|---|
expression of mutant C47S | Streptococcus pyogenes |
Crystallization (Comment) | Organism |
---|---|
purified recombinant mutant C47S, X-ray diffraction crystal structure determination and analysis at 1.6 A resolution | Streptococcus pyogenes |
Protein Variants | Comment | Organism |
---|---|---|
C47S | crystal structure analysis | Streptococcus pyogenes |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
x * 40000, immature zymogen, x * 28000, mature processed enzyme | Streptococcus pyogenes |
40000 | - |
x * 40000, immature zymogen, x * 28000, mature processed enzyme | Streptococcus pyogenes |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Streptococcus pyogenes | streptopain shows strepadhesin activity, independent of protease activity, mediated by a cell surface adhesin and controlled by the multiple gene regulator Mga | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus pyogenes | - |
the enzyme is expressed in all strains | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | the enzyme performs autolytic processing to the mature 28 kDa protein by removal of an about 117 amino-acid peptide from the N-terminus | Streptococcus pyogenes |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
preferential cleavage with hydrophobic residues at P2, P1 and P1' | the catalytic residue is His195 forming an ion pair with the single Cys47 residue | Streptococcus pyogenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Fibronectin + H2O | - |
Streptococcus pyogenes | ? | - |
? | |
human interleukin 1beta precursor + H2O | - |
Streptococcus pyogenes | human interleukin 1beta + human interleukin 1beta signal peptide | - |
? | |
insulin beta chain + H2O | reduced and carboxylated substrate, cleavage of the linkage Phe25-Tyr26 | Streptococcus pyogenes | ? | - |
? | |
additional information | streptopain shows strepadhesin activity, independent of protease activity, mediated by a cell surface adhesin and controlled by the multiple gene regulator Mga | Streptococcus pyogenes | ? | - |
? | |
additional information | cleavage site specificity, overview, the enzyme prefers cleavage of Phe-Tyr bonds, the enzyme shows poor activity with trypsin substrates fibrin, casein, and gelatin from human and rabbit | Streptococcus pyogenes | ? | - |
? | |
Vitronectin + H2O | - |
Streptococcus pyogenes | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000, immature zymogen, x * 28000, mature processed enzyme | Streptococcus pyogenes |
More | primary, and tertiary structure | Streptococcus pyogenes |
Synonyms | Comment | Organism |
---|---|---|
interleukin-1beta convertase | - |
Streptococcus pyogenes |
More | the enzyme belongs to the peptidase family C10 | Streptococcus pyogenes |
pyrogenic exotoxin B | - |
Streptococcus pyogenes |
SpeB | - |
Streptococcus pyogenes |
Streptococcal cysteine proteinase | - |
Streptococcus pyogenes |
streptococcal proteinase | - |
Streptococcus pyogenes |
Streptococcus peptidase A | - |
Streptococcus pyogenes |