Cloned (Comment) | Organism |
---|---|
expression of the prostasin proenzyme in Escherichia coli as insoluble inclusion bodies, refolding and activating via proteolytic removal of the N-terminal propeptide | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method. Active prostasin is crystallized, and the structure is determined to 1.45 A resolution. These apoprotein crystals are soaked with nafamostat, allowing the structure of the inhibited acyl-enzyme intermediate structure to be determined to 2.0 A resolution | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0335 | - |
acetyl-KHYR-7-amido-4-methylcoumarin | pH 9, 23°C, variant 801 expressed in Sf21 cells, glycosylated | Homo sapiens | |
0.0343 | - |
acetyl-KHYR-7-amido-4-methylcoumarin | pH 9, 23°C, variant 28 expressed in Escherichia coli, not glycosylated | Homo sapiens | |
0.0388 | - |
acetyl-KHYR-7-amido-4-methylcoumarin | pH 9, 23°C, variant 26 expressed in Escherichia coli, not glycosylated | Homo sapiens | |
0.04 | - |
acetyl-KHYR-7-amido-4-methylcoumarin | pH 9, 23°C, variant 40 expressed in Sf21 cells, not glycosylated | Homo sapiens | |
0.043 | - |
acetyl-KHYR-7-amido-4-methylcoumarin | pH 9, 23°C, variant 35 expressed in Sf21 cells, glycosylated | Homo sapiens | |
0.0493 | - |
acetyl-KHYR-7-amido-4-methylcoumarin | pH 9, 23°C, variant 1 expressed in mouse cells, glycosylated | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24000 | - |
purified refolded prostasin, gel filtration | Homo sapiens |
27879 | - |
1 * 27879, refolded and purified prostasin variant 26, calculated from sequence | Homo sapiens |
28351 | - |
1 * 28351, refolded and purified prostasin variant 28, calculated from sequence | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q16651 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-KHYR-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | acetyl-KHYR + 7-amino-4-methylcoumarin | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 27879, refolded and purified prostasin variant 26, calculated from sequence | Homo sapiens |
monomer | 1 * 28351, refolded and purified prostasin variant 28, calculated from sequence | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
acetyl-KHYR-7-amido-4-methylcoumarin | pH 9, 23°C, variant 26 expressed in Escherichia coli, not glycosylated | Homo sapiens | |
0.13 | - |
acetyl-KHYR-7-amido-4-methylcoumarin | pH 9, 23°C, variant 40 expressed in Sf21 cells, not glycosylated | Homo sapiens | |
0.14 | - |
acetyl-KHYR-7-amido-4-methylcoumarin | pH 9, 23°C, variant 28 expressed in Escherichia coli, not glycosylated | Homo sapiens | |
0.17 | - |
acetyl-KHYR-7-amido-4-methylcoumarin | pH 9, 23°C, variant 35 expressed in Sf21 cells, glycosylated | Homo sapiens | |
0.23 | - |
acetyl-KHYR-7-amido-4-methylcoumarin | pH 9, 23°C, variant 801 expressed in Sf21 cells, glycosylated | Homo sapiens | |
0.27 | - |
acetyl-KHYR-7-amido-4-methylcoumarin | pH 9, 23°C, variant 1 expressed in mouse cells, glycosylated | Homo sapiens |