Crystallization (Comment) | Organism |
---|---|
mutant E32A, W129A and S278A pro-kumamolisin are crystallized by sitting-drop vapor-diffusion method, 1.2/1.3 A crystal structure | Bacillus novosp. |
Protein Variants | Comment | Organism |
---|---|---|
E32A | the ratio of turnover number to Km-value for the substrate Lys-Pro-Ile-Ala-Phe-(4-nitro)Phe-Arg-Leu is reduced to 5.7% of the native enzyme | Bacillus novosp. |
S278A | the ratio of turnover number to Km-value for the substrate Lys-Pro-Ile-Ala-Phe-(4-nitzo)Phe-Arg-Leu is reduced to 0.5% of the native enzyme | Bacillus novosp. |
W129A | the ratio of turnover number to Km-value for the substrate Lys-Pro-Ile-Ala-Phe-(4-nitro)Phe-Arg-Leu is reduced to 3.8% of the native enzyme | Bacillus novosp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.029 | - |
Lys-Pro-Ile-Ala-Phe-(4-nitro)Phe-Arg-Leu | pH 3.5, 60°C, wild-type enzyme | Bacillus novosp. | |
0.084 | - |
Lys-Pro-Ile-Ala-Phe-(4-nitro)Phe-Arg-Leu | pH 3.5, 60°C, mutant enzyme S278A | Bacillus novosp. | |
0.089 | - |
Lys-Pro-Ile-Ala-Phe-(4-nitro)Phe-Arg-Leu | pH 3.5, 60°C, mutant enzyme W129A | Bacillus novosp. | |
0.102 | - |
Lys-Pro-Ile-Ala-Phe-(4-nitro)Phe-Arg-Leu | pH 3.5, 60°C, mutant enzyme E32A | Bacillus novosp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Bacillus novosp. | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus novosp. | - |
MN-32 | - |
Bacillus novosp. MN-32 | - |
MN-32 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | pro-kumamolisinS278A: the prodomain exhibits a half-beta sandwich core docking to the catalytic domain similarly as the equivalent subtilisin prodomain complexes | Bacillus novosp. |
Purification (Comment) | Organism |
---|---|
- |
Bacillus novosp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Lys-Pro-Ile-Ala-Phe-(4-nitro)Phe-Arg-Leu + H2O | - |
Bacillus novosp. | Lys-Pro-Ile-Ala-Phe + Nph-Arg-Leu | - |
? | |
Lys-Pro-Ile-Ala-Phe-(4-nitro)Phe-Arg-Leu + H2O | - |
Bacillus novosp. MN-32 | Lys-Pro-Ile-Ala-Phe + Nph-Arg-Leu | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
Lys-Pro-Ile-Ala-Phe-(4-nitro)Phe-Arg-Leu | pH 3.5, 60°C, wild-type enzyme | Bacillus novosp. | |
0.08 | - |
Lys-Pro-Ile-Ala-Phe-(4-nitro)Phe-Arg-Leu | pH 3.5, 60°C, mutant enzyme S278A | Bacillus novosp. | |
0.67 | - |
Lys-Pro-Ile-Ala-Phe-(4-nitro)Phe-Arg-Leu | pH 3.5, 60°C, mutant enzyme W129A | Bacillus novosp. | |
1.24 | - |
Lys-Pro-Ile-Ala-Phe-(4-nitro)Phe-Arg-Leu | pH 3.5, 60°C, mutant enzyme E32A | Bacillus novosp. | |
6.02 | - |
Lys-Pro-Ile-Ala-Phe-(4-nitro)Phe-Arg-Leu | pH 3.5, 60°C, wild-type enzyme | Bacillus novosp. | |
6.08 | - |
Lys-Pro-Ile-Ala-Phe-(4-nitro)Phe-Arg-Leu | pH 3.5, 60°C, mutant enzyme E32A | Bacillus novosp. | |
6.08 | - |
Lys-Pro-Ile-Ala-Phe-(4-nitro)Phe-Arg-Leu | pH 3.5, 60°C, mutant enzyme W129A | Bacillus novosp. |