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Literature summary for 3.4.21.B40 extracted from

  • Filippou, P.S.; Farkona, S.; Brinc, D.; Yu, Y.; Prassas, I.; Diamandis, E.P.
    Biochemical and functional characterization of the human tissue kallikrein 9 (2017), Biochem. J., 474, 2417-2433 .
    View publication on PubMed
Search for more literature for 3.4.21.B40

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Expi293 cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Aprotinin 16% inhibition at 0.001 mM Homo sapiens
leupeptin 38% inhibition at 0.001 mM Homo sapiens
additional information EDTA alone does not have any inhibitory effect on the enzyme activity Homo sapiens
phenylmethylsulfonyl fluoride almost complete inhibition at 10 mM Homo sapiens
serpin A3 most potent serpin inhibitor, complete inhibition at 0.001 mM Homo sapiens
serpin A5 about 40% inhibition at 0.001 mM Homo sapiens
serpin C1 about 60% inhibition at 0.001 mM Homo sapiens
serpin F2 about 35% inhibition at 0.001 mM Homo sapiens
Zn2+ reversible inhibition. 5 mM ZnCl2 almost completely abolishes the enzyme activity. EDTA (final concentration of 50 mM) completely reverses the Zn2+ inhibition Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
 LLVY-7-amido-4-methylcoumarin at pH 8.0 and 37°C Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ about 180% activity at 25 mM Homo sapiens
Mg2+ about 220% activity at 12.5 mM Homo sapiens
additional information Na+ has no significant effect on the enzyme activity Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9UKQ9
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
Mono Q column chromatography and Viva C4 column chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
HaCaT cell
-
 Homo sapiens
-
SCC-9 cell
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.298
-
 with LLVY-7-amido-4-methylcoumarin as substrate, at pH 8.0 and 37°C Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
AAPF-7-amido-4-methylcoumarin + H2O 15% activity compared to LLVY-7-amido-4-methylcoumarin Homo sapiens AAPF + 7-amino-4-methylcoumarin
-
 ?
LLVY-7-amido-4-methylcoumarin + H2O preferred substrate Homo sapiens LLVY + 7-amino-4-methylcoumarin
-
 ?
midkine + H2O
-
 Homo sapiens ?
-
 ?
additional information the enzyme has strong preference for tyrosine over phenylalanine at the P1 cleavage position Homo sapiens ?
-
 ?
tissue kallikrein 10 + H2O
-
 Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
? x * 25000, deglycosylated protein, SDS-PAGE Homo sapiens
? x * 27500, calculated from amino acid sequence Homo sapiens
? x * 42000, glycosylated protein, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
KLK9
-
 Homo sapiens
tissue kallikrein 9
-
 Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 at 37°C, the enzyme retains about 75% and 50% activity after 6-12 h and 48 h, respectively Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
7 10 more than 40% activity between pH 7.0 and 10.0 Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.34
-
 LLVY-7-amido-4-methylcoumarin at pH 8.0 and 37°C Homo sapiens