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Literature summary for 3.4.21.B39 extracted from

  • Yousef, G.M.; Kapadia, C.; Polymeris, M.E.; Borgono, C.; Hutchinson, S.; Wasney, G.A.; Soosaipillai, A.; Diamandis, E.P.
    The human kallikrein protein 5 (hK5) is enzymatically active, glycosylated and forms complexes with two protease inhibitors in ovarian cancer fluids (2003), Biochim. Biophys. Acta, 1628, 88-96.
    View publication on PubMed
Search for more literature for 3.4.21.B39

Application

Application Comment Organism
medicine regulation by protease inhibitors may have potential clinical applications Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
DNA sequence determination and analysis, expression in CHO-CCL-61 cells, in Escherichia coli, and in Pichia pastoris Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
alpha1-antitrypsin
-
 Homo sapiens
alpha2-Macroglobulin
-
 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
 recombinant deglycosylated enzyme, gel filtration Homo sapiens
30000
-
 recombinant glycosylated protein, gel filtration Homo sapiens
40000
-
 native glycosylated enzyme, gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens the enzyme is differentially expressed at the mRNA and protein level in diverse malignancies including cancer ?
-
 ?
polypeptide + H2O Homo sapiens enzyme is regulated by binding to protease inhibitors peptides
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9Y337 gene KLK5
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein N-glycosylated Homo sapiens
glycoprotein 4 potential N-glycosylation sites at amino acid residues positions 69, 173, 208, and 252 Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from CHO-CCL-61 cells and from Pichia pastoris Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
proteolytic cleavage of polypeptides enzyme is a serine protease Homo sapiens
a

Source Tissue

Source Tissue Comment Organism Textmining
ascites fluid from ovarian cancer patients Homo sapiens
-
milk
-
 Homo sapiens
-
serum female and male Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
FSR-amido-4-methylcoumarin + H2O fluorogenic trypsin-like peptide substrate Homo sapiens FSR + 7-amino-4-methylcoumarin
-
 ?
additional information no activity with AAPF-amido-4-methylcoumarin Homo sapiens ?
-
 ?
additional information enzyme shows trypsin-like but not chymotrypsin-like activity Homo sapiens ?
-
 ?
additional information the enzyme is differentially expressed at the mRNA and protein level in diverse malignancies including cancer Homo sapiens ?
-
 ?
polypeptide + H2O
-
 Homo sapiens peptides
-
 ?
polypeptide + H2O enzyme is regulated by binding to protease inhibitors Homo sapiens peptides
-
 ?
VPR-amido-4-methylcoumarin + H2O fluorogenic trypsin-like peptide substrate Homo sapiens VPR + 7-amino-4-methylcoumarin
-
 ?

Synonyms

Synonyms Comment Organism
K5
-
 Homo sapiens
kallikrein protein 5
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Homo sapiens