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Literature summary for 3.4.21.B30 extracted from

  • Chaurasiya, K.R.; Ruslie, C.; Silva, M.C.; Voortman, L.; Nevin, P.; Lone, S.; Beuning, P.J.; Williams, M.C.
    Polymerase manager protein UmuD directly regulates Escherichia coli DNA polymerase III alpha binding to ssDNA (2013), Nucleic Acids Res., 41, 8959-8968.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.21.B30

Cloned(Commentary)

Cloned (Comment) Organism
gene umuD, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21 (DE3) Escherichia coli

Protein Variants

Protein Variants Comment Organism
D91K site-directed mutagenesis, the mutation abolishes the interaction between the enzyme and the DNA polymerase III alpha subunit Escherichia coli
G92K site-directed mutagenesis, the mutation abolishes the interaction between the enzyme and the DNA polymerase III alpha subunit Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Escherichia coli enzyme UmuD does not bind DNA. the enzyme UmuD interacts with several components of DNA polymerase III, including the polymerase subunit alpha, the beta clamp and the proofreading subunit epsilon, homology modeling and protein-protein docking analysis, overview. It interacts with the alpha subunit of DNA polymerase III at two distinct binding sites, one of which is adjacent to the single-stranded DNA-binding site. Enzyme UmuD specifically inhibits binding of DNA polymerase III alpha to ssDNA, UmuD residues D91 and G92 are involved in this interaction, molecular modeling, overview ?
-
 ?
UmuD + H2O Escherichia coli auto-cleavage of UmuD to UmuD' UmuD' + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme UmuD does not bind DNA. the enzyme UmuD interacts with several components of DNA polymerase III, including the polymerase subunit alpha, the beta clamp and the proofreading subunit epsilon, homology modeling and protein-protein docking analysis, overview. It interacts with the alpha subunit of DNA polymerase III at two distinct binding sites, one of which is adjacent to the single-stranded DNA-binding site. Enzyme UmuD specifically inhibits binding of DNA polymerase III alpha to ssDNA, UmuD residues D91 and G92 are involved in this interaction, molecular modeling, overview Escherichia coli ?
-
 ?
additional information enzyme UmuD does not bind DNA Escherichia coli ?
-
 ?
UmuD + H2O auto-cleavage of UmuD to UmuD' Escherichia coli UmuD' + ?
-
 ?

Synonyms

Synonyms Comment Organism
UmuD
-
 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Escherichia coli

General Information

General Information Comment Organism
metabolism competition between enzyme UmuD and ssDNA for DNA polymerase III alpha binding is a distinct mechanism for polymerase exchange Escherichia coli
physiological function the protein UmuD is extensively involved in modulating cellular responses to DNA damage and may play a role in DNA polymerase exchange for damage tolerance. The polymerase manager protein UmuD directly regulates Escherichia coli DNA polymerase III alpha binding to ssDNA Escherichia coli