Crystallization (Comment) | Organism |
---|---|
hanging-drop vapour-diffusion method, protein solution: 10 mg/ml, 50 mM K/Na phosphate, pH 6.7, 1 mM NaN3, 10-14% polyethylene glycol 3350, 4°C, three-dimensional structure determination by X-ray diffraction and analysis | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.087 | - |
LVTQEVSPKIVGGS | pH 8.0, 37°C | Bos taurus | |
0.2 | - |
alpha-N-t-butoxycarbonyl-Gly-Phe-Arg-4-nitroanilide | pH 8.0, 37°C | Bos taurus | |
0.39 | - |
alpha-N-succinyl-Ser-Thr-Pro-Lys-4-nitroanilide | pH 8.0, 37°C | Bos taurus | |
0.54 | - |
alpha-N-benzoyl-Val-Gly-Arg-4-nitroanilide | pH 8.0, 37°C | Bos taurus | |
1 | - |
alpha-N-succinyl-Ser-Pro-Lys-4-nitroanilide | pH 8.0, 37°C | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
Golgi apparatus | - |
Bos taurus | 5794 | - |
rough endoplasmic reticulum | - |
Bos taurus | 5791 | - |
secretory granule | - |
Bos taurus | 30141 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
polypeptide + H2O | Bos taurus | - |
peptides | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | P80219 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
Brunner's gland | excretory duct | Bos taurus | - |
duodenum | - |
Bos taurus | - |
epithelium | secretory cells of Brunner's gland | Bos taurus | - |
mucosa | - |
Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-N-benzoyl-Val-Gly-Arg-4-nitroanilide + H2O | - |
Bos taurus | alpha-N-benzoyl-Val-Gly-Arg + 4-nitroaniline | - |
? | |
alpha-N-succinyl-Ser-Pro-Lys-4-nitroanilide + H2O | - |
Bos taurus | alpha-N-succinyl-Ser-Pro-Lys + 4-nitroaniline | - |
? | |
alpha-N-succinyl-Ser-Thr-Pro-Lys-4-nitroanilide + H2O | - |
Bos taurus | alpha-N-succinyl-Ser-Thr-Pro-Lys + 4-nitroaniline | - |
? | |
alpha-N-succinyl-Thr-Pro-Lys-4-nitroanilide + H2O | - |
Bos taurus | alpha-N-succinyl-Thr-Pro-Lys + 4-nitroaniline | - |
? | |
alpha-N-t-butoxycarbonyl-Gly-Phe-Arg-4-nitroanilide + H2O | - |
Bos taurus | N-t-butoxycarbonyl-Gly-Phe-Arg + 4-nitroaniline | - |
? | |
alpha-N-tosyl-Gly-Pro-Lys-4-nitroanilide + H2O | - |
Bos taurus | alpha-N-tosyl-Gly-Pro-Lys + 4-nitroaniline | - |
? | |
angiotensin II + H2O | enzyme cleaves bond between residues 2-3 and 4-5 | Bos taurus | fragments of angiotensin II | - |
? | |
angiotensinogen-(1-14) + H2O | enzyme cleaves bond between residues 8-9 | Bos taurus | fragments of angiotensinogen-(1-14) | - |
? | |
bovine duodenase + H2O | enzyme cleaves bond between residues 14-15, 17-18, 63-64, 70-71, 110-111, 138-139, 157-158, 162-163, 174-175 and 197-198 | Bos taurus | fragments of bovine duodenase | - |
? | |
bovine serum albumin + H2O | enzyme cleaves bond between residues 27-28, 41-42, 64-65, 210-211, 223-224, 231-232, 238-239, and 409-410 | Bos taurus | fragments of bovine serum albumin | - |
? | |
Escherichia coli La-protease + H2O | enzyme cleaves bond between residues 219-220 and 239-240 | Bos taurus | fragments of Escherichia coli La-protease | - |
? | |
glucagon + H2O | enzyme cleaves bond between residues 12-13 and 18-19 | Bos taurus | fragments of glucagon | - |
? | |
human gamma-interferon + H2O | enzyme cleaves bond between residues 69-70 | Bos taurus | fragments of human gamma-interferon | - |
? | |
human interleukin II-(59-79) + H2O | enzyme cleaves bond between residues 66-67 | Bos taurus | fragments of human interleukin II-(59-79) | - |
? | |
LVTQEVSPKIVGGS + H2O | - |
Bos taurus | LVTQEVSPK + IVGGS | - |
? | |
melittin + H2O | enzyme cleaves bond between residues 6-7, 7-8, 9-10, and 22-23 | Bos taurus | fragments of melittin | - |
? | |
Naja naja oxiana neurotoxin II + H2O | enzyme cleaves bond between residues 15-16, 26-27, 44-45, and 32-33 | Bos taurus | fragments of Naja naja oxiana neurotoxin II | - |
? | |
polypeptide + H2O | - |
Bos taurus | peptides | - |
? | |
porcine proinsulin + H2O | enzyme cleaves bond between residues 29-30, 31-32, 32-33, 56-57, 64-65 and 66-67 | Bos taurus | fragments of porcine proinsulin | - |
? | |
surfagon + H2O | enzyme cleaves bond between residues 3-4 | Bos taurus | fragments of surfagon | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Bos taurus |
Synonyms | Comment | Organism |
---|---|---|
duodenase I | - |
Bos taurus |
duodenase serine protease | - |
Bos taurus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Bos taurus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6 | - |
alpha-N-benzoyl-Val-Gly-Arg-4-nitroanilide | pH 8.0, 37°C | Bos taurus | |
24 | - |
alpha-N-t-butoxycarbonyl-Gly-Phe-Arg-4-nitroanilide | pH 8.0, 37°C | Bos taurus | |
84 | - |
LVTQEVSPKIVGGS | pH 8.0, 37°C | Bos taurus | |
156 | - |
alpha-N-succinyl-Ser-Pro-Lys-4-nitroanilide | pH 8.0, 37°C | Bos taurus | |
438 | - |
alpha-N-succinyl-Ser-Thr-Pro-Lys-4-nitroanilide | pH 8.0, 37°C | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Bos taurus |