BRENDA - Enzyme Database
show all sequences of 3.4.21.B26

Deletion of the gene encoding proprotein convertase 5/6 causes early embryonic lethality in the mouse

Essalmani, R.; Hamelin, J.; Marcinkiewicz, J.; Chamberland, A.; Mbikay, M.; Chretien, M.; Seidah, N.G.; Prat, A.; Mol. Cell. Biol. 26, 354-361 (2006)

Data extracted from this reference:

Engineering
Amino acid exchange
Commentary
Organism
additional information
Pcsk5delta4-deficient allele missing exon 4 that encodes the catalytic Asp173, delta4/delta4 genotype is embryonic lethal, heterozygote mice are healthy and fertile
Mus musculus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
PC5B-isoform
Mus musculus
16020
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
110000
-
Western blot analysis, PC5A isoform
Mus musculus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
Q92824
-
-
Mus musculus
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
3T3-L1 cell
-
Mus musculus
-
A-431 cell
-
Homo sapiens
-
A-549 cell
-
Homo sapiens
-
adrenal cortex
richest source of PC5A
Mus musculus
-
adult
-
Mus musculus
-
aorta
PC5A dominates
Mus musculus
-
brain cortex
PC5A dominates
Mus musculus
-
CACO-2 cell
-
Homo sapiens
-
cerebellum
PC5A dominates
Mus musculus
-
colon
PC5B dominates
Mus musculus
-
duodenum
PC5B dominates
Mus musculus
-
embryo
-
Mus musculus
-
heart
PC5A dominates
Mus musculus
-
HEK-293 cell
-
Homo sapiens
-
HeLa cell
-
Homo sapiens
-
Hep-G2 cell
-
Homo sapiens
-
hippocampus
furture hippocampus, CA3 region
Mus musculus
-
HT-1080 cell
-
Homo sapiens
-
HT-29 cell
-
Homo sapiens
-
Huh-7 cell
-
Homo sapiens
-
HUVEC cell
-
Homo sapiens
-
ileum
PC5B dominates
Mus musculus
-
incisor
-
Mus musculus
-
intestine
richest source of PC5B
Mus musculus
-
jejunum
PC5B dominates
Mus musculus
-
JURKAT cell
-
Homo sapiens
-
kidney
PC5B dominates
Mus musculus
-
liver
both isoforms equally expressed
Mus musculus
-
LoVo cell
-
Homo sapiens
-
lung
PC5A dominates
Mus musculus
-
MCF-7 cell
-
Homo sapiens
-
additional information
NCI-H146 cell line, NCI-H345 cell line, NCI-A520 cell line, LoVo-C5 cell line, BON-1 cell line
Homo sapiens
-
additional information
first bronchial arch, wall of bulbus cordis, the nasal pit and pituitary, alphaT3-1 cell line, betaT3-1 cell line, AfT-20 cell line, CT26 cell line, CG-2 cell line, TM3-cell line, Ltk cell line and ES cell line
Mus musculus
-
muscle
PC5A dominates
Mus musculus
-
Neuro-2A cell
-
Mus musculus
-
NIH-3T3 cell
-
Mus musculus
-
ovary
PC5A dominates
Mus musculus
-
primordium
pancreatic and adrenal primordium
Mus musculus
-
somite
-
Mus musculus
-
spleen
PC5A dominates
Mus musculus
-
stomach
PC5A dominates
Mus musculus
-
testis
PC5A dominates
Mus musculus
-
umbilical cord
-
Mus musculus
-
uterus
PC5A dominates
Mus musculus
-
Y-1 cell
most highly expressing PC5 line
Mus musculus
-
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
Pcsk5delta4-deficient allele missing exon 4 that encodes the catalytic Asp173, delta4/delta4 genotype is embryonic lethal, heterozygote mice are healthy and fertile
Mus musculus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
PC5B-isoform
Mus musculus
16020
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
110000
-
Western blot analysis, PC5A isoform
Mus musculus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
3T3-L1 cell
-
Mus musculus
-
A-431 cell
-
Homo sapiens
-
A-549 cell
-
Homo sapiens
-
adrenal cortex
richest source of PC5A
Mus musculus
-
adult
-
Mus musculus
-
aorta
PC5A dominates
Mus musculus
-
brain cortex
PC5A dominates
Mus musculus
-
CACO-2 cell
-
Homo sapiens
-
cerebellum
PC5A dominates
Mus musculus
-
colon
PC5B dominates
Mus musculus
-
duodenum
PC5B dominates
Mus musculus
-
embryo
-
Mus musculus
-
heart
PC5A dominates
Mus musculus
-
HEK-293 cell
-
Homo sapiens
-
HeLa cell
-
Homo sapiens
-
Hep-G2 cell
-
Homo sapiens
-
hippocampus
furture hippocampus, CA3 region
Mus musculus
-
HT-1080 cell
-
Homo sapiens
-
HT-29 cell
-
Homo sapiens
-
Huh-7 cell
-
Homo sapiens
-
HUVEC cell
-
Homo sapiens
-
ileum
PC5B dominates
Mus musculus
-
incisor
-
Mus musculus
-
intestine
richest source of PC5B
Mus musculus
-
jejunum
PC5B dominates
Mus musculus
-
JURKAT cell
-
Homo sapiens
-
kidney
PC5B dominates
Mus musculus
-
liver
both isoforms equally expressed
Mus musculus
-
LoVo cell
-
Homo sapiens
-
lung
PC5A dominates
Mus musculus
-
MCF-7 cell
-
Homo sapiens
-
additional information
NCI-H146 cell line, NCI-H345 cell line, NCI-A520 cell line, LoVo-C5 cell line, BON-1 cell line
Homo sapiens
-
additional information
first bronchial arch, wall of bulbus cordis, the nasal pit and pituitary, alphaT3-1 cell line, betaT3-1 cell line, AfT-20 cell line, CT26 cell line, CG-2 cell line, TM3-cell line, Ltk cell line and ES cell line
Mus musculus
-
muscle
PC5A dominates
Mus musculus
-
Neuro-2A cell
-
Mus musculus
-
NIH-3T3 cell
-
Mus musculus
-
ovary
PC5A dominates
Mus musculus
-
primordium
pancreatic and adrenal primordium
Mus musculus
-
somite
-
Mus musculus
-
spleen
PC5A dominates
Mus musculus
-
stomach
PC5A dominates
Mus musculus
-
testis
PC5A dominates
Mus musculus
-
umbilical cord
-
Mus musculus
-
uterus
PC5A dominates
Mus musculus
-
Y-1 cell
most highly expressing PC5 line
Mus musculus
-
Other publictions for EC 3.4.21.B26
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
731128
Heng
Development of a high-throughp ...
Homo sapiens
Anal. Biochem.
475
14-21
2015
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2
2
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731111
Lee
Proprotein convertase 5/6A is ...
Homo sapiens
Am. J. Respir. Cell Mol. Biol.
52
749-761
2014
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1
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1
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2
1
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732515
Paule
Proprotein convertase 5/6 clea ...
Homo sapiens
Mol. Hum. Reprod.
21
262-270
2014
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1
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1
2
2
1
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731899
Paule
Cleavage of endometrial alpha- ...
Homo sapiens
Hum. Reprod.
27
2766-2774
2012
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1
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1
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2
1
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4
4
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717191
Kim
Galectin-3 binding protein pro ...
Homo sapiens
Biochem. Biophys. Res. Commun.
404
96-102
2011
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1
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6
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6
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718009
Fritzsche
Time for endothelial cell prop ...
Mus musculus
J. Mol. Med.
89
1061-1063
2011
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6
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2
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1
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7
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6
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7
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1
1
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698386
Fuller
Subtilisin-like proprotein con ...
Homo sapiens
Invest. Ophthalmol. Vis. Sci.
50
5759-5768
2009
-
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-
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1
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709911
Sun
The proprotein convertase PC5/ ...
Mus musculus
Mol. Cancer
8
73
2009
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4
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7
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7
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2
2
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680190
Seidah
The activation and physiologic ...
Mus musculus
Int. J. Biochem. Cell Biol.
40
1111-1125
2008
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1
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680909
Mayer
The regulated cell surface zym ...
Mus musculus
J. Biol. Chem.
283
2373-2384
2008
1
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1
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1
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1
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5
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5
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691487
Padua
Regulation of DNA synthesis an ...
Mus musculus
BMC Cell Biol.
9
5
2008
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693094
Remacle
Substrate cleavage analysis of ...
Homo sapiens
J. Biol. Chem.
283
20897-20906
2008
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694893
Essalmani
In vivo functions of the propr ...
Mus musculus
Proc. Natl. Acad. Sci. USA
105
5750-5755
2008
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695251
Xiao
Cell-surface processing of ext ...
Mus musculus
Virology
372
384-397
2008
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679779
Dikeakos
PC1/3, PC2 and PC5/6A are targ ...
Mus musculus
FEBS J.
274
4094-4102
2007
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682018
Fugere
Short polybasic peptide sequen ...
Homo sapiens
Mol. Pharmacol.
71
323-332
2007
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1
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8
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8
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675967
Essalmani
Deletion of the gene encoding ...
Homo sapiens, Mus musculus
Mol. Cell. Biol.
26
354-361
2006
-
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1
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1
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1
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3
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45
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1
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1
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45
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678042
Stein
RNAi-mediated silencing of pro ...
Mus musculus
Biochem. J.
400
209-215
2006
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1
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680696
Benjannet
The proprotein convertase (PC) ...
Homo sapiens
J. Biol. Chem.
281
30561-30572
2006
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Seidah
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Proprotein convertase 5 ...
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