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Literature summary for 3.4.21.B21 extracted from

  • Godovikova, V.; Wang, H.T.; Goetting-Minesky, M.P.; Ning, Y.; Capone, R.F.; Slater, C.K.; Fenno, J.C.
    Treponema denticola PrcB is required for expression and activity of the PrcA-PrtP (dentilisin) complex (2010), J. Bacteriol., 192, 3337-3344.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.21.B21

Cloned(Commentary)

Cloned (Comment) Organism
His-tagged enzyme is expressed in Escherichia coli JM109 cells Treponema denticola

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane outer membrane Treponema denticola 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
PrcA + H2O Treponema denticola
-
 PrcA1 + PrcA2 two auxillary polypeptides ?

Organism

Organism UniProt Comment Textmining
Treponema denticola
-
 strain ATCC 35405
-

Purification (Commentary)

Purification (Comment) Organism
nickel affinity column chromatography Treponema denticola

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
PrcA + H2O
-
 Treponema denticola PrcA1 + PrcA2 two auxillary polypeptides ?
succinyl-L-alanyl-L-alanyl-L-prolyl-L-phenylalanine-4-nitroanilide + H2O
-
 Treponema denticola succinyl-L-alanyl-L-alanyl-L-prolyl-L-phenylalanine + 4-nitroaniline
-
 ?

Synonyms

Synonyms Comment Organism
dentilisin
-
 Treponema denticola
PrtP protease
-
 Treponema denticola

Expression

Organism Comment Expression
Treponema denticola PrcB is required for native expression of PrtP, PrcB is a PrtP-binding lipoprotein that stabilizes the PrtP polypeptide during localization to the outer membrane up