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Literature summary for 3.4.21.B12 extracted from

  • Takayama, T.K.; McMullen, B.A.; Nelson, P.S.; Matsumura, M.; Fujikawa, K.
    Characterization of hK4 (prostase), a prostate-specific serine protease: activation of the precursor of prostate specific antigen (pro-PSA) and single-chain urokinase-type plasminogen activator and degradation of prostatic acid phosphatase (2001), Biochemistry, 40, 15341-15348.
    View publication on PubMed
Search for more literature for 3.4.21.B12

Cloned(Commentary)

Cloned (Comment) Organism
construction of a chimeric enzyme with exchange of the pro-piece of the zymogen for that of prostate-specific antigen PSA to create an activation site suceptible for trypsin-type proteases, expression in Escherichia coli strain BL21(DE3) Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Aprotinin complex formation with the self-activated recombinant, chimeric enzyme Homo sapiens
benzamidine 93% inhibition at 10 mM Homo sapiens
Nalpha-p-tosyl-L-lysine chloromethyl ketone 85% inhibition at 1 mM; i.e. TLCK Homo sapiens
Soybean trypsin inhibitor 99% inhibition at 1 mg/ml Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
21000
-
 x * 21000, self-activated recombinant, chimeric enzyme, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
52 kDa prostatic acid phosphatase + H2O Homo sapiens enzyme may be involved in the physiological clearance of prostatic acid phosphatase 33 kDa prostatic acid phosphatase + 19 kDa fragment
-
 ?
polypeptide + H2O Homo sapiens enzyme plays a role in the physiologic processing of seminal plasma proteins , e.g. pro-PSA, as well as in the pathogenesis of prostate cancer through the activation of pro-PSA peptides
-
 ?
pro-prostate-specific antigen + H2O Homo sapiens i.e. PSA activated prostate-specific antigen
-
 ?
pro-prostate-specific antigen + H2O Homo sapiens activation of PSA activated prostate-specific antigen
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification recombinant chimeric enzyme with exchange of the pro-piece of the zymogen for that of prostate-specific antigen PSA to create an activation site suceptible for trypsin-type proteases proteolytically activates itself Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
recombinant chimeric enzyme after isolation from inclusion bodies and refolding Homo sapiens

Renatured (Commentary)

Renatured (Comment) Organism
isolation from inclusion bodies after recombinant expression in Escherichia coli, refolding with self-activation during the process Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
prostate specific Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.31
-
 purified recombinant enzyme, substrate H-D-Val-L-Leu-L-Lys-4-nitroanilide Homo sapiens
0.67
-
 purified recombinant enzyme, substrate N-benzoyl-L-Ile-L-Glu-Gly-L-Arg-4-nitroanilide Homo sapiens
1.12
-
 purified recombinant enzyme, substrate H-D-Pro-L-Phe-L-Arg-4-nitroanilide Homo sapiens
1.42
-
 purified recombinant enzyme, substrate H-D-Val-Leu-Arg-4-nitroanilide Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
52 kDa prostatic acid phosphatase + H2O recombinant self-activated, chimeric enzyme Homo sapiens 33 kDa prostatic acid phosphatase + 19 kDa fragment products contain 256 and 73 amino acid residues, respectively ?
52 kDa prostatic acid phosphatase + H2O primary cleavage site is the Arg73-Ser74 bond Homo sapiens 33 kDa prostatic acid phosphatase + 19 kDa fragment products contain 256 and 73 amino acid residues, respectively ?
52 kDa prostatic acid phosphatase + H2O inactivation of substrate Homo sapiens 33 kDa prostatic acid phosphatase + 19 kDa fragment products contain 256 and 73 amino acid residues, respectively ?
52 kDa prostatic acid phosphatase + H2O enzyme may be involved in the physiological clearance of prostatic acid phosphatase Homo sapiens 33 kDa prostatic acid phosphatase + 19 kDa fragment
-
 ?
benzoyl-L-Ile-L-Glu-Gly-L-Arg-4-nitroanilide + H2O recombinant self-activated, chimeric enzyme Homo sapiens benzoyl-L-Ile-L-Glu-Gly-L-Arg + 4-nitroaniline
-
 ?
benzoyl-L-Ile-L-Glu-Gly-L-Arg-4-nitroanilide + H2O fluorogenic substrate, i.e. S-2222 Homo sapiens benzoyl-L-Ile-L-Glu-Gly-L-Arg + 4-nitroaniline
-
 ?
D-Pro-L-Phe-L-Arg-4-nitroanilide + H2O recombinant self-activated, chimeric enzyme Homo sapiens D-Pro-L-Phe-L-Arg + 4-nitroaniline
-
 ?
D-Pro-L-Phe-L-Arg-4-nitroanilide + H2O fluorogenic substrate, i.e. S-2302 Homo sapiens D-Pro-L-Phe-L-Arg + 4-nitroaniline
-
 ?
D-Val-L-Leu-L-Lys-4-nitroanilide + H2O recombinant self-activated, chimeric enzyme Homo sapiens D-Val-L-Leu-L-Lys + 4-nitroaniline
-
 ?
D-Val-L-Leu-L-Lys-4-nitroanilide + H2O fluorogenic substrate, i.e. S-2251 Homo sapiens D-Val-L-Leu-L-Lys + 4-nitroaniline
-
 ?
D-Val-Leu-Arg-4-nitroanilide + H2O recombinant self-activated, chimeric enzyme Homo sapiens D-Val-Leu-Arg + 4-nitroaniline
-
 ?
D-Val-Leu-Arg-4-nitroanilide + H2O fluorogenic substrate, i.e. S-2266 Homo sapiens D-Val-Leu-Arg + 4-nitroaniline
-
 ?
additional information no activity with serum albumin from human seminal plasma Homo sapiens ?
-
 ?
additional information enzyme has a trypsin-type substrate specificty Homo sapiens ?
-
 ?
additional information no activity with pyroglu-Gly-Arg-4-nitroanilide, i.e. S-2444, and with 3-carbomethoxypropionyl-L-Arg-L-Pro-L-Tyr-4-nitroanilide, i.e. S-2586 Homo sapiens ?
-
 ?
polypeptide + H2O
-
 Homo sapiens peptides
-
 ?
polypeptide + H2O enzyme plays a role in the physiologic processing of seminal plasma proteins , e.g. pro-PSA, as well as in the pathogenesis of prostate cancer through the activation of pro-PSA Homo sapiens peptides
-
 ?
pro-prostate-specific antigen + H2O activation of substrate Homo sapiens activated prostate-specific antigen
-
 ?
pro-prostate-specific antigen + H2O recombinant self-activated, chimeric enzyme Homo sapiens activated prostate-specific antigen
-
 ?
pro-prostate-specific antigen + H2O i.e. PSA Homo sapiens activated prostate-specific antigen
-
 ?
pro-prostate-specific antigen + H2O activation of PSA Homo sapiens activated prostate-specific antigen
-
 ?
pro-urokinase-type plasminogen activator + H2O activation of substrate Homo sapiens activated urokinase-type plasminogen activator
-
 ?
pro-urokinase-type plasminogen activator + H2O recombinant self-activated, chimeric enzyme Homo sapiens activated urokinase-type plasminogen activator
-
 ?
pro-urokinase-type plasminogen activator + H2O i.e. pro-uPA Homo sapiens activated urokinase-type plasminogen activator
-
 ?

Subunits

Subunits Comment Organism
? x * 21000, self-activated recombinant, chimeric enzyme, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
K4
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
5 10.3 7% of maximal activity at ph 5.0, 32% at pH 5.5, and 40% at pH 10.3 Homo sapiens