Cloned (Comment) | Organism |
---|---|
the precursor form of human kallikrein 6 is overexpressed in Pichia pastoris and is autoprocessed to an active but unstable mature enzyme that subsequently yields the inactive, self-cleavage product, hK6(D81-K244) | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
R80Q | mutation stabilizes the activity of the mature enzyme | Homo sapiens |
S197A | mutation results in complete loss of hK6 proteolytic activity | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Bovine pancreatic trypsin inhibitor | weak | Homo sapiens | |
Bowman-Birk inhibitor | weak | Homo sapiens | |
Soybean trypsin inhibitor | weak | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
plasminogen + H2O | Homo sapiens | the enzyme is regulated by an autoactivation/autoinactivation mechanism. Mature hK67 displays a trypsin-like activity against human plasminogen, the putative physiological substrate | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Gelatin + H2O | - |
Homo sapiens | ? | - |
? | |
additional information | hK6 does not hydrolyze the Nalpha-tosyl-L-Arg methyl ester | Homo sapiens | ? | - |
? | |
Nalpha-benzoyl-L-Arg + H2O | - |
Homo sapiens | benzoic acid + L-Arg | - |
? | |
plasminogen + H2O | - |
Homo sapiens | ? | - |
? | |
plasminogen + H2O | the enzyme is regulated by an autoactivation/autoinactivation mechanism. Mature hK67 displays a trypsin-like activity against human plasminogen, the putative physiological substrate | Homo sapiens | ? | - |
? |