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Literature summary for 3.4.21.B1 extracted from
- Activation of proPHBSP, the zymogen of a plasma hyaluronan binding serine protease, by an intermolecular autocatalytic mechanism (2000), Biol. Chem., 381, 1223-1231.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Dextran sulfate | strongly accelerates autoactivation, strongest acceleration at 0.02 mg/ml of 5-kDa-dextran sulfate | Homo sapiens | |
| heparin | strongly accelerates autoactivation, optimum concentration: 5-10 U/ml, presence of 7.5 U/ml reduces the half-life of the proenzyme from 12 min to 3.5 min | Homo sapiens |  |
| poly-L-lysine | strongly accelerates autoactivation | Homo sapiens | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| alpha2 antiplasmin | inhibits autoactivation at physiological concentrations | Homo sapiens | |
| Aprotinin | potent serine protease inhibitor, inhibits autoactivation at physiological concentrations | Homo sapiens | |
| C1 esterase inhibitor | inhibits autoactivation at physiological concentrations | Homo sapiens | |
| PPACK | potent serine protease inhibitor, inhibits autoactivation at physiological concentrations | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 70000 | - |
proenzyme, SDS-PAGE | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Protein + H2O | Homo sapiens | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| proteolytic modification | autocatalytic activation | Homo sapiens |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| purification with 6M urea yields the single-chain zymogen, whereas native conditions yield the activated two-chain form | Homo sapiens |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| complete recovery of enzyme activity within 2 min of incubation at 1 M urea after denaturation in 6M urea | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| plasma | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| coagulation factor FVII + H2O | - |
Homo sapiens | ? | - |
? | |
| factor V + H2O | - |
Homo sapiens | ? | - |
? | |
| factor VIII + H2O | - |
Homo sapiens | ? | - |
? | |
| L-pyroglutamic acid-cyclo-hexylglycine-Arg-para-nitroanilide + H2O | P-3342, fluorogenic substrate | Homo sapiens | ? | - |
? | |
| plasminogen activator precursor + H2O | - |
Homo sapiens | ? | - |
? | |
| Protein + H2O | - |
Homo sapiens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PHBSP | - |
Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Homo sapiens |
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