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Literature summary for 3.4.21.96 extracted from

  • Guo, T.; Ouyang, X.; Xin, Y.; Wang, Y.; Zhang, S.; Kong, J.
    Characterization of a new cell envelope proteinase PrtP from Lactobacillus rhamnosus CGMCC11055 (2016), J. Agric. Food Chem., 64, 6985-6992 .
    View publication on PubMed
Search for more literature for 3.4.21.96

Inhibitors

Inhibitors Comment Organism Structure
Ba2+ 1 mM, 82% of initial activity Lacticaseibacillus rhamnosus
Cu2+ 1 mM, 77% of initial activity Lacticaseibacillus rhamnosus
EDTA 1 mM, 45% of initial activity Lacticaseibacillus rhamnosus
EGTA 1 mM, 52% of initial activity Lacticaseibacillus rhamnosus
Fe2+ 1 mM, 82% of initial activity Lacticaseibacillus rhamnosus
K+ 1 mM, 79% of initial activity Lacticaseibacillus rhamnosus
Li+ 1 mM, 89% of initial activity Lacticaseibacillus rhamnosus
Mn2+ 1 mM, 78% of initial activity Lacticaseibacillus rhamnosus
p-amidinophenylmethylsulfonyl fluoride
-
 Lacticaseibacillus rhamnosus
PMSF 1 mM, 12% residual activity Lacticaseibacillus rhamnosus
Sn2+ 1 mM, 77% of initial activity Lacticaseibacillus rhamnosus
Sr2+ 1 mM, 93% of initial activity Lacticaseibacillus rhamnosus

Localization

Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 1 mM, 106% of initial activity Lacticaseibacillus rhamnosus
Co2+ 1 mM, 112% of initial activity Lacticaseibacillus rhamnosus
Mg2+ 1 mM, 109% of initial activity Lacticaseibacillus rhamnosus
Zn2+ 1 mM, 108% of initial activity Lacticaseibacillus rhamnosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
170000
-
 above 170000 Da, PAGE Lacticaseibacillus rhamnosus

Organism

Organism UniProt Comment Textmining
Lacticaseibacillus rhamnosus
-
-
-
Lacticaseibacillus rhamnosus CGMCC11055
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Lacticaseibacillus rhamnosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alphas1-casein + H2O
-
 Lacticaseibacillus rhamnosus ?
-
 ?
alphas1-casein + H2O
-
 Lacticaseibacillus rhamnosus CGMCC11055 ?
-
 ?
beta-casein + H2O preferred substrate Lacticaseibacillus rhamnosus ?
-
 ?
beta-casein + H2O preferred substrate Lacticaseibacillus rhamnosus CGMCC11055 ?
-
 ?
additional information proline is the preferred residue at the cleavage site. No substrate: kappa-casein Lacticaseibacillus rhamnosus ?
-
 ?
additional information proline is the preferred residue at the cleavage site. No substrate: kappa-casein Lacticaseibacillus rhamnosus CGMCC11055 ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * more than 170000 Da, PAGE Lacticaseibacillus rhamnosus

Synonyms

Synonyms Comment Organism
PrtP
-
 Lacticaseibacillus rhamnosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
44
-
-
 Lacticaseibacillus rhamnosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 30 min, pH 6.5, stable Lacticaseibacillus rhamnosus
50
-
 30 min, pH 6.5, 20% residual activity Lacticaseibacillus rhamnosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Lacticaseibacillus rhamnosus