Activating Compound | Comment | Organism | Structure |
---|---|---|---|
PC1/3 CT peptide | increases PC1/3 activity at low concentrations (nanomol range). Interaction with the active site of PC1/3 does not result in the formation of a stable complex. Activation of PC1/3 by 5 nanomol CT-peptide is the same in the presence or absence of propeptide | Mus musculus |
Application | Comment | Organism |
---|---|---|
additional information | PC1/3, through its various domains, is capable of controlling its enzymatic activity in all regions of the cell that it encounters | Mus musculus |
Cloned (Comment) | Organism |
---|---|
PC1/3 produced using the baculovirus expression system in Sf9 insect cells or through intracoelemic injection in insect larvae. PC1/3 CT-peptide from positions 592-726 cloned into a pet24b+ bacterial expression vector. The resulting C-terminally His-tagged protein expressed in Escherichia coli strain BL21 (DE3) | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
PC1/3 CT peptide | decreases PC1/3 activity at high concentrations (micromol range) | Mus musculus | |
PC1/3 propeptide | 20 nanomol leads to a 50% reduction in enzymatic activity | Mus musculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
17640 | - |
mass spectrometry | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant PC1/3 purified. PC1/3 CT peptide purified by His-affinity chromatography and RP-HPLC | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | PC1/3 CT peptide is not cleaved by enzymatically active PC1/3 | Mus musculus | ? | - |
? | |
pGlu-Arg-Thr-Lys-Arg-7-amido-4-methylcoumarin + H2O | - |
Mus musculus | pGlu-Arg-Thr-Lys-Arg + 7-amino-4-methylcoumarin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PC1/3 | - |
Mus musculus |
proprotein convertase 1/3 | - |
Mus musculus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
at pH 7.8 increase of enzymatic activity up to 50-60%, irrespective of the amount of PC1/3 CT peptide used up to 0.005 mM | Mus musculus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
PC1/3 CT peptide | - |
Mus musculus |