Inhibitors | Comment | Organism | Structure |
---|---|---|---|
antipain | 0.1 mM, no residual activity | Leishmania donovani | |
benzamidine | 1 mM, 27% residual activity | Leishmania donovani | |
Ca2+ | 0.01 mM, 69% residual activity | Leishmania donovani | |
leupeptin | 0.1 mM, no residual activity | Leishmania donovani | |
Mg2+ | 0.01 mM, 73% residual activity | Leishmania donovani | |
Mn2+ | 0.01 mM, 73% residual activity | Leishmania donovani | |
Pefabloc SC | 2 mM, no residual activity | Leishmania donovani | |
Phe-Pro-Arg-chloromethylketone | 0.01 mM, no residual activity | Leishmania donovani | |
tosyl-L-Lys-chloromethylketone | 0.1 mM, no residual activity | Leishmania donovani | |
Zn2+ | 0.01 mM, no residual activity | Leishmania donovani |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00308 | - |
benzoyl-Gly-Gly-L-Arg-7-amido-4-methylcoumarin | pH 8.0, 25°C | Leishmania donovani | |
0.00776 | - |
benzoyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin | pH 8.0, 25°C | Leishmania donovani |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania donovani | C9EF60 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzoyl-Gly-Gly-L-Arg-7-amido-4-methylcoumarin + H2O | - |
Leishmania donovani | benzoyl-Gly-Gly-L-Arg + 7-amino-4-methylcoumarin | - |
? | |
benzoyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin + H2O | - |
Leishmania donovani | benzoyl-Gly-L-Pro-L-Arg + 7-amino-4-methylcoumarin | - |
? | |
additional information | OPB has a strong preference for lysine or arginine residues at P1. OPB can accommodate several amino acids in the P2P4 positions, with a preference for glycine in P2, serine, glycine, alanine, asparagine, proline, and threonine in P3, and proline in P4. Bulky hydrophobic groups, such as tyrosine, phenylalanine, and tryptophan, are least preferred | Leishmania donovani | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4250 | - |
benzoyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin | pH 8.0, 25°C | Leishmania donovani | |
6830 | - |
benzoyl-Gly-Gly-L-Arg-7-amido-4-methylcoumarin | pH 8.0, 25°C | Leishmania donovani |
General Information | Comment | Organism |
---|---|---|
physiological function | knock-out of OPB results in the disappearance of the serine protease activity of Leishmania extracts. OPB null parasites show an elevated content of enolase protein. This enolase is enzymatically inactive and associated with the parasite membrane. OPB deletion results in a striking alteration in macrophage responses to Leishmania. Whereas wild type parasites elicited little, if any, response from infected macrophages, OPB deletion parasites induce a massive up-regulation in gene transcription. These OPB deletion parasites display decreased virulence in the murine footpad indection model | Leishmania donovani |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
547700 | - |
benzoyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin | pH 8.0, 25°C | Leishmania donovani | |
2218000 | - |
benzoyl-Gly-Gly-L-Arg-7-amido-4-methylcoumarin | pH 8.0, 25°C | Leishmania donovani |