Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetyl-L-Leu-L-Lys-L-Arg-4-nitroanilide | - |
Serratia proteamaculans | |
benzoyl-L-Pro-L-Phe-L-Arg-4-nitroanilide | - |
Serratia proteamaculans | |
Nalpha-benzyloxycarbonyl-L-Phe-L-Arg-4-nitroanilide | - |
Serratia proteamaculans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0226 | - |
acetyl-L-Leu-L-Lys-L-Arg-4-nitroanilide | pH 8.0, 25°C | Serratia proteamaculans | |
0.0513 | - |
Nalpha-benzyloxycarbonyl-L-Phe-L-Arg-4-nitroanilide | presence of 50 mM Ca2+, pH 8.0, 25°C | Serratia proteamaculans | |
0.058 | - |
Nalpha-benzyloxycarbonyl-L-Phe-L-Arg-4-nitroanilide | pH 8.0, 25°C | Serratia proteamaculans | |
0.084 | - |
benzoyl-L-Pro-L-Phe-L-Arg-4-nitroanilide | presence of 50 mM Ca2+, pH 8.0, 25°C | Serratia proteamaculans | |
0.09 | - |
Nalpha-benzoyl-DL-arginine-4-nitroanilide | pH 8.0, 25°C | Serratia proteamaculans | |
0.096 | - |
Nalpha-benzyloxycarbonyl-L-lysine thiobenzyl ester | pH 8.0, 25°C | Serratia proteamaculans | |
0.224 | - |
acetyl-L-Leu-L-Leu-L-Arg-4-nitroanilide | pH 8.0, 25°C | Serratia proteamaculans | |
0.265 | - |
acetyl-L-Leu-L-Lys-L-Arg-4-nitroanilide | presence of 50 mM Ca2+, pH 8.0, 25°C | Serratia proteamaculans | |
0.302 | - |
Nalpha-benzoyl-DL-arginine-4-nitroanilide | presence of 50 mM Ca2+, pH 8.0, 25°C | Serratia proteamaculans | |
0.306 | - |
succinyl-L-AlaL-Ala-L-Pro-L-Arg-4-nitroanilide | presence of 50 mM Ca2+, pH 8.0, 25°C | Serratia proteamaculans | |
0.322 | - |
Nalpha-benzyloxycarbonyl-L-lysine thiobenzyl ester | presence of 50 mM Ca2+, pH 8.0, 25°C | Serratia proteamaculans | |
0.457 | - |
acetyl-L-Leu-L-Leu-L-Arg-4-nitroanilide | presence of 50 mM Ca2+, pH 8.0, 25°C | Serratia proteamaculans | |
0.54 | - |
benzoyl-L-Pro-L-Phe-L-Arg-4-nitroanilide | pH 8.0, 25°C | Serratia proteamaculans | |
1.05 | - |
succinyl-L-AlaL-Ala-L-Pro-L-Arg-4-nitroanilide | pH 8.0, 25°C | Serratia proteamaculans | |
2.2 | - |
succinyl-L-Ala-L-Ala-L-Pro-L-Lys-4-nitroanilide | presence of 50 mM Ca2+, pH 8.0, 25°C | Serratia proteamaculans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | in presence of Ca2+, efficiency of hydrolysis of substrates with positively charged P2 residues decreases by an order of magnitude due to the worsening in the substrate binding. In the presence of 50 mM Ca2+, no substrate inhibition is observed in the case of acetyl-L-Leu-L-Lys-L-Arg-4-nitroanilide, and the effect was less pronounced for other substrates | Serratia proteamaculans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
78000 | - |
x * 78000, SDS-PAGE | Serratia proteamaculans |
Organic Solvent | Comment | Organism |
---|---|---|
dimethyl sulfoxide | assay in presence of 2% dimethyl sulfoxide | Serratia proteamaculans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Serratia proteamaculans | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-L-Leu-L-Leu-L-Arg-4-nitroanilide + H2O | - |
Serratia proteamaculans | acetyl-L-Leu-L-Leu-L-Arg + 4-nitroaniline | - |
? | |
acetyl-L-Leu-L-Lys-L-Arg-4-nitroanilide + H2O | - |
Serratia proteamaculans | acetyl-L-Leu-L-Lys-L-Arg + 4-nitroaniline | - |
? | |
benzoyl-L-Pro-L-Phe-L-Arg-4-nitroanilide + H2O | - |
Serratia proteamaculans | benzoyl-L-Pro-L-Phe-L-Arg + 4-nitroaniline | - |
? | |
Nalpha-benzoyl-DL-arginine-4-nitroanilide + H2O | - |
Serratia proteamaculans | Nalpha-benzoyl-DL-arginine + 4-nitroaniline | - |
? | |
Nalpha-benzyloxycarbonyl-L-Ala-L-Ala-L-Arg-4-nitroanilide + H2O | poor substrate | Serratia proteamaculans | Nalpha-benzyloxycarbonyl-L-Ala-L-Ala-L-Arg + 4-nitroaniline | - |
? | |
Nalpha-benzyloxycarbonyl-L-lysine thiobenzyl ester + H2O | - |
Serratia proteamaculans | Nalpha-benzyloxycarbonyl-L-lysine + phenylmethanethiol | - |
? | |
Nalpha-benzyloxycarbonyl-L-Phe-L-Arg-4-nitroanilide + H2O | - |
Serratia proteamaculans | Nalpha-benzyloxycarbonyl-L-Phe-L-Arg + 4-nitroaniline | - |
? | |
succinyl-Gly-L-Pro-L-Lys-4-nitroanilide + H2O | poor substrate | Serratia proteamaculans | succinyl-Gly-L-Pro-L-Lys + 4-nitroaniline | - |
? | |
succinyl-L-Ala-L-Ala-L-Pro-L-Lys-4-nitroanilide + H2O | - |
Serratia proteamaculans | succinyl-L-Ala-L-Ala-L-Pro-L-Lys + 4-nitroaniline | - |
? | |
succinyl-L-AlaL-Ala-L-Pro-L-Arg-4-nitroanilide + H2O | - |
Serratia proteamaculans | succinyl-L-Ala-L-Ala-L-Pro-L-Arg + 4-nitroaniline | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 78000, SDS-PAGE | Serratia proteamaculans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Serratia proteamaculans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
rapid loss of activity above | Serratia proteamaculans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
succinyl-L-AlaL-Ala-L-Pro-L-Arg-4-nitroanilide | pH 8.0, 25°C | Serratia proteamaculans | |
1 | - |
succinyl-L-Ala-L-Ala-L-Pro-L-Lys-4-nitroanilide | presence of 50 mM Ca2+, pH 8.0, 25°C | Serratia proteamaculans | |
1.2 | - |
succinyl-L-AlaL-Ala-L-Pro-L-Arg-4-nitroanilide | presence of 50 mM Ca2+, pH 8.0, 25°C | Serratia proteamaculans | |
2 | 8 | Nalpha-benzyloxycarbonyl-L-Phe-L-Arg-4-nitroanilide | pH 8.0, 25°C | Serratia proteamaculans | |
7.6 | - |
acetyl-L-Leu-L-Leu-L-Arg-4-nitroanilide | pH 8.0, 25°C | Serratia proteamaculans | |
13.9 | - |
acetyl-L-Leu-L-Leu-L-Arg-4-nitroanilide | presence of 50 mM Ca2+, pH 8.0, 25°C | Serratia proteamaculans | |
14.5 | - |
Nalpha-benzoyl-DL-arginine-4-nitroanilide | pH 8.0, 25°C | Serratia proteamaculans | |
21.2 | - |
benzoyl-L-Pro-L-Phe-L-Arg-4-nitroanilide | presence of 50 mM Ca2+, pH 8.0, 25°C | Serratia proteamaculans | |
24.3 | - |
acetyl-L-Leu-L-Lys-L-Arg-4-nitroanilide | pH 8.0, 25°C | Serratia proteamaculans | |
32 | - |
acetyl-L-Leu-L-Lys-L-Arg-4-nitroanilide | presence of 50 mM Ca2+, pH 8.0, 25°C | Serratia proteamaculans | |
35.2 | - |
Nalpha-benzyloxycarbonyl-L-Phe-L-Arg-4-nitroanilide | presence of 50 mM Ca2+, pH 8.0, 25°C | Serratia proteamaculans | |
37.7 | - |
Nalpha-benzoyl-DL-arginine-4-nitroanilide | presence of 50 mM Ca2+, pH 8.0, 25°C | Serratia proteamaculans | |
53.8 | - |
Nalpha-benzyloxycarbonyl-L-lysine thiobenzyl ester | presence of 50 mM Ca2+, pH 8.0, 25°C | Serratia proteamaculans | |
55.5 | - |
Nalpha-benzyloxycarbonyl-L-lysine thiobenzyl ester | pH 8.0, 25°C | Serratia proteamaculans | |
201.7 | - |
benzoyl-L-Pro-L-Phe-L-Arg-4-nitroanilide | pH 8.0, 25°C | Serratia proteamaculans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Serratia proteamaculans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9.5 | - |
Serratia proteamaculans |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.027 | - |
benzoyl-L-Pro-L-Phe-L-Arg-4-nitroanilide | pH 8.0, 25°C | Serratia proteamaculans | |
0.045 | - |
Nalpha-benzyloxycarbonyl-L-Phe-L-Arg-4-nitroanilide | pH 8.0, 25°C | Serratia proteamaculans | |
0.126 | - |
Nalpha-benzyloxycarbonyl-L-Phe-L-Arg-4-nitroanilide | presence of 50 mM Ca2+, pH 8.0, 25°C | Serratia proteamaculans | |
0.19 | - |
benzoyl-L-Pro-L-Phe-L-Arg-4-nitroanilide | presence of 50 mM Ca2+, pH 8.0, 25°C | Serratia proteamaculans | |
0.4 | - |
acetyl-L-Leu-L-Lys-L-Arg-4-nitroanilide | pH 8.0, 25°C | Serratia proteamaculans |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
succinyl-L-Ala-L-Ala-L-Pro-L-Lys-4-nitroanilide | pH 8.0, 25°C | Serratia proteamaculans | |
0.4 | - |
succinyl-L-Ala-L-Ala-L-Pro-L-Lys-4-nitroanilide | presence of 50 mM Ca2+, pH 8.0, 25°C | Serratia proteamaculans | |
0.5 | - |
succinyl-L-AlaL-Ala-L-Pro-L-Arg-4-nitroanilide | pH 8.0, 25°C | Serratia proteamaculans | |
2 | - |
Nalpha-benzyloxycarbonyl-L-lysine thiobenzyl ester | presence of 50 mM Ca2+, pH 8.0, 25°C | Serratia proteamaculans | |
3 | - |
succinyl-L-AlaL-Ala-L-Pro-L-Arg-4-nitroanilide | presence of 50 mM Ca2+, pH 8.0, 25°C | Serratia proteamaculans | |
5.8 | - |
Nalpha-benzyloxycarbonyl-L-lysine thiobenzyl ester | pH 8.0, 25°C | Serratia proteamaculans | |
10.8 | - |
acetyl-L-Leu-L-Lys-L-Arg-4-nitroanilide | pH 8.0, 25°C | Serratia proteamaculans | |
30 | - |
acetyl-L-Leu-L-Leu-L-Arg-4-nitroanilide | presence of 50 mM Ca2+, pH 8.0, 25°C | Serratia proteamaculans | |
34 | - |
acetyl-L-Leu-L-Leu-L-Arg-4-nitroanilide | pH 8.0, 25°C | Serratia proteamaculans | |
120 | - |
acetyl-L-Leu-L-Lys-L-Arg-4-nitroanilide | presence of 50 mM Ca2+, pH 8.0, 25°C | Serratia proteamaculans | |
125 | - |
Nalpha-benzoyl-DL-arginine-4-nitroanilide | presence of 50 mM Ca2+, pH 8.0, 25°C | Serratia proteamaculans | |
161 | - |
Nalpha-benzoyl-DL-arginine-4-nitroanilide | pH 8.0, 25°C | Serratia proteamaculans | |
251 | - |
benzoyl-L-Pro-L-Phe-L-Arg-4-nitroanilide | presence of 50 mM Ca2+, pH 8.0, 25°C | Serratia proteamaculans | |
377 | - |
benzoyl-L-Pro-L-Phe-L-Arg-4-nitroanilide | pH 8.0, 25°C | Serratia proteamaculans | |
485 | - |
Nalpha-benzyloxycarbonyl-L-Phe-L-Arg-4-nitroanilide | pH 8.0, 25°C | Serratia proteamaculans | |
680 | - |
Nalpha-benzyloxycarbonyl-L-Phe-L-Arg-4-nitroanilide | presence of 50 mM Ca2+, pH 8.0, 25°C | Serratia proteamaculans |